Pathogenicity of a Strain of Streptococcus suis Type 2 to Mice and Swine Isolated from Jingzhou City

Main Article Content

Mengjie Liu
Zongming Li
Xiaolin Yang
Guoping Liu

Abstract

Aims: The objective of this study is to identify S. suis type 2 and evaluate the virulence of ZHJ01 strain isolation, and verity the clinical and pathological outcome of a systemic infection caused by one serotype 2 when simultaneously inoculated with ZHJ01 strain. We also want to clarify the epidemiologic, clinical, microbiologic characteristics and the pathogenesis mechanism of S. suis type 2 in Hubei province, China.


Study Design: Pigs suspected of being infected with S. suis in Jingzhou regions of Hubei province, China were studied. S. suis type 2 isolation was obtained from the suspicion of diseased pig. The case of S. suis type 2 was detected by the virulence factor amplification based on PCR detection and bacterial isolation, identification in the laboratory. According to the experimental infections of mice and piglets, pathogenicity of this S. suis type 2 isolation to mice and swine was monitored. This study was conducted in the key laboratory of pathogenic microbiology, College of Animal Science of Yangtze University, and Institute of Black Pigs Research, Yangtze University.


Methodology: Proper serological typing can be performed using a co-agglutination test. The typical colonies purificated and cultured were inoculated with Glucose, Lactose, Raffinos, Sorbitol, D (+)-sucrose, Trehalose, 6.5%NaCl, D (-)-Salicin, Hippurate, Esculin hydrate, V-p, etc., then the test results were recorded. Detection of virulence factors were performed using PCR amplification and DNA sequencing. S.suis type 2 isolation was inoculated to mice and piglets for the virulence test, and the observation of the clinical signs and pathological changes.


Results: The virulence factor of extracellular protein factor (EF) was determined from ZHJ01 strain based on PCR detection. Sequence analysis indicated that the isolate was very similar to nucleotide homology with others SS2 strains from different county or contries, and there was not much variation. LD50 of S. suis type 2 for mice was 2.5 x 107cfu. LD50 of S. suis type 2 for piglets was 3.92 x 109cfu.


Conclusion: The results show that Swine S. suis type 2 has a relatively strong pathogenicity to pigs in Hubei province, China. This study can be, in part, sufficient to explain the pathogenicity for ZHJ01 strain in area of Zhijing, Jingzhou city, China, which may provide insights into the pathogenesis SS2 and more valid data to support the development of S. suis vaccine as well as the epidemiological investigation, further monitoring and effective prevention to S. suis.

Keywords:
Streptococcus suis type 2, identification, biological trait, experimental model, median lethal dose (LD50)

Article Details

How to Cite
Liu, M., Li, Z., Yang, X., & Liu, G. (2019). Pathogenicity of a Strain of Streptococcus suis Type 2 to Mice and Swine Isolated from Jingzhou City. Biotechnology Journal International, 23(2), 1-12. https://doi.org/10.9734/bji/2019/v23i230072
Section
Original Research Article

References

Gottschalk M, Zimmerman J, Karriker L, Ramirez A, Schwarz K, Stevenson G, Eds. Streptococcosis in diseases of swine, 10th Edition. Wiley-Blackwell: West Sussex, UK. 2012;841–855.

Gottschalk M, Xu J, Calzas C, Segura M. Streptococcus suis: A new emerging or an old neglected zoonotic pathogen. Future Microbiol. 2010;5:371–391.

Fittipaldi N, Segura M, Grenier D, Gottschalk M. Virulence factors involved in the pathogenesis of the infection caused by the swine pathogen and zoonotic agent Streptococcus suis. Future Microbiol. 2012;7:259–279.

Dutkiewicz J, Sroka J, Zając V, Wasiński B, Cisak E, Sawczyn A, Kloc A, Wójcik-Fatla A. Streptococcus suis: A re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I. Epidemiology. Ann Agric Environ Med. 2017;24(4):683–695.

Fittipaldi N, Segura M, Grenier D, Gottschalk M. Virulence factors involved in the pathogenesis of the infection caused by the swine pathogen and zoonotic agent S.suis. Future microbiology. 2012;7(2): 259–279.

Epub 2012/02/14.

DOI: 10.2217/fmb.11.149 PMID: 22324994

Segura M, Fittipaldi N, Calzas C, Gottschalk M. Critical Streptococcus suis virulence factors: Are they all really critical? Trends Microbiol. 2017;25:585–599.

Segura M, Calzas C, Grenier D, Gottschalk M. Initial steps of the pathogenesis of the infection caused by Streptococcus suis: Fighting against nonspecific defenses. FEBS Lett. 2016;590:3772–3799.

Marini E, Palmieri C, Magi G, Facinelli B. Recombination between S. suis ICESsu32457 and Streptococcus agalactiae ICESa2603 yields a hybrid ICE transferable to Streptococcus pyogenes. Veterinary microbiology. 2015;178(1–2): 99–104.

Epub 2015/05/04.

DOI:10.1016/j.vetmic.2015.04.013

PMID: 25935120.

Zhang XL, Shen QF, Zhen XT, et al. Identification of S. suis and establishment of PCR type method. Heilong jiang Animal Science and Veterinary Medicine. 2005;9: 86-87.

Holden MT, Hauser H, Sanders M, et al. Rapid evolution of virulence and drug resistance in the emerging zoonotic pathogen S. suis. PLoS One. 2009;60-72.

Fittipaldi N, Segura M, Grenier D, et al. Virulence factors involved in the pathogenesis of the infection caused by the swine pathogen and zoonotic agent Streptococcus suis [J]. Future Microbiology. 2012;7(2):259-279.

Kim SG, Kim YH, Lee HY, et al. PFGE patterns of Streptococcus suis isolates from diseased pigs in Gyeongbuk province, Korea [J]. Korean Journal of Veterinary Service. 2012;35(4).

Hu XS, Zhu FC, Wang H, et al. Studies on human streptococcal infectious syndrome caused by infected pigs, Chinese Journal of Preventive Medicine. 2000;24(3):150-152.

Zhao Y, et al. Role of a type IV-like secretion system of Streptococcus suis 2 in the development of streptococcal toxic shock syndrome. J Infect Dis. 2011;204: 274–281.

Chen C, Jiaqi T, Wei D, et al. A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 chinese isolates [J]. PLoS ONE. 2007;2(3):e315.

Shi-Dan T, Guo-Ping L, Bioengineering DO. Identification and biological characteristic of Streptococcus suis type 2 in Hubei [J]. Hubei Agricultural Sciences; 2017.

Gottschalk M, Higgins R, Boudreau M. Use of polyvalent co-agglutination reagents for serotyping of Streptococcus suis [J]. Journal of Clinical Microbiology. 1993; 31(8):2192.

Charland N, Harel J, Kobisch M, Lacasse S, Gottschalk M. Streptococcus suis serotype 2 mutants deficient in capsular expression. Microbiology. 1998;144(Pt 2): 325–332.

Zhijie L, Han Z, Marcelo G, et al. Development of Multiplex PCR Assays for the Identification of the 33 Serotypes of Streptococcus suis [J]. PLoS ONE. 2013; 8(8):e72070.

Holden MT, Hauser H, Sanders M, Ngo TH, Cherevach I, Cronin A, Goodhead I, Mungall K, Quail MA, Price C, et al. Rapid evolution of virulence and drug resistance in the emerging zoonotic pathogen Streptococcus suis. PLoS One. 2009; 4(7):e6072.

Chen C, Tang J, Dong W, Wang C, Feng Y, Wang J, Zheng F, Pan X, Liu D, Li M, et al. A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates. PLoS ONE. 2007;2(3):e315.

Yao X, Li M, Wang J, et al. Isolation and characterization of a native avirulent strain of Streptococcus suis serotype 2: A perspective for vaccine development [J]. Scientific Reports. 2015;5:9835.

Marois C, Bougeard S, Gottschalk M, et al. Multiplex PCR assay for detection of Streptococcus suis species and serotypes 2 and 1/2 in tonsils of live and dead pigs [J]. Journal of Clinical Microbiology. 2004; 42(7):3169-75.

Zhao Z, Wang J, Liu P, et al. Cultivation, LD50 determination and experimental model of Streptococcus suis serotype 2 strain HA9801 [J]. Research in Veterinary Science. 2009;86(2):200-205.

Blume V, Luque I, Vela AI, et al. Genetic and virulence-phenotype characterization of serotypes 2 and 9 of Swine Streptococcus suis isolates [J]. International Microbiology the Official Journal of the Spanish Society for Microbiology. 2009;12(3):161.

Reed LJ, Muench HA. A simple method of estimating fifty percent endpoints. A. J. Trop. Med. Hyg. 1938;27:493–497.

Zheng H, Ji S, Lan R, et al. Population analysis of Streptococcus suis isolates from slaughtered swine by use of minimum core genome sequence typing [J]. Journal of Clinical Microbiology. 2014;52(10): 3568-3572.

Hu J, You W, Wang B, et al. Construction, characterization and evaluation of the protective efficacy of the Streptococcus suis double mutant strain ΔSsPep/ ΔSsPspC as a live vaccine candidate in mice [J]. Microbiological Research. 2015; 170:87-94.

Zhao Z, Wang J, Liu P, et al. Cultivation, LD50 determination and experimental model of Streptococcus suis serotype 2 strain HA9801[J]. Research in Veterinary Science. 2009;86(2):200-205.

Wisselink HJ, Engel B, Smith HE. Detection of extracellular factor-positive Streptococcus suis serotype 2 strains in tonsillar swabs of live sows by PCR [J]. Veterinary Microbiology. 2005;109(3):223-228.

Wen-Jie G, Ping-Ping Y, Han-Ping Z. Sequence analysis of two virulence factors from Streptococcus suis type 2 isolated in Jiaxing, Zhejiang province [J]. Chinese Journal of Public Health. 2010;31(1):107.

Domínguez-Punaro MC, Segura M, Plante M-M, Lacouture S, Rivest S, Gottschalk M. Streptococcus suis serotype 2, an important swine and human pathogen, induces strong systemic and cerebral inflammatory responses in a mouse model of infection. J Immunol. 2007;179: 1842–54.

Bi Y, Li J, Yang L, Zhang S, Li Y, Jia X, Liu W. Assessment of the pathogenesis of Streptococcus suis type 2 infection in piglets for understanding streptococcal toxic shock-like syndrome, meningitis, and sequelae. Veterinary Microbiology. 2014; 173(3-4):299–309.

DOI: 10.1016/j. vetmic. 2014.08.010

Herman V, Faur B, Pascu C, et al. Characterization of some Streptococcus suis strains isolated from pigs [J]; 2011.

Dominguez-Punaro MC, Segura M, Plante MM, Lacouture S, Rivest S, et al. Streptococcus suis serotype 2, an important swine and human pathogen, induces strong systemic and cerebral inflammatory responses in a mouse model of infection. J Immunol. 2007;179:1842– 1854.

Gottschalk M. Streptococcosis. pp. 841-855. In: Disease of Swine, 10th ed. (Straw, B. E., Zimmerman, J. J., D'Allaire, S. and Taylor, D. J. eds.), Blackwell Publishing Professional, Ames; 2012.

Feng Y, Zhang H, Wu Z, Wang S, Cao M, Hu D, Wang C. Streptococcus suis infection: An emerging / reemerging challenge of bacterial infectious diseases? Virulence. 2014;5:477–497.

Knuradeszczka S, Lipperheide C, Petersen B, et al. Plasma haptoglobin concentration in swine after challenge with Streptococcus suis[J]. Zoonoses & Public Health. 2010; 49(5):240-244.

Segura M. Streptococcus suis vaccines: candidate antigens and progress. Expert Rev Vaccines. 2015;14:1587-608.