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The study was carried out for callus induction and synthetic seed development from the shoot tips of Draceana sanderiana sander ex Mast. The shoot tips were subjected to different concentrations (0.25, 0.5 &1.0 mg/l) of 2,4-D on MS medium. The research findings revealed that the 2,4-D at concentrations of 0.25 mg/l was more suited for the profuse callus formation. The friable and light yellow callus was induced within 2 weeks of culture at 0.25 mg/l of 2,4-D on MS medium as compared to the other two concentrations of 2,4-D i.e.; 0.5 and 1.0 mg/l. Similarly the effect of sodium alginate and calcium chloride percentage on synthetic seed formation was observed, it was found that somatic embryos formed from shoot tips via callus kept in 2.5% sodium alginate and 100 milli molar CaCl2 produced synthetic seeds with firm spherical beads. The study leads to the formation of synthetic seeds of Draceana sanderiana which can be used for the conservation of germplasm through cryopreservation and the micro propagation of the said plant species.
Okunji CO, Iwn MM, Jackson JE, Tally JD. Biological activity of saponins from two Dracaena species. Adv. Exp. Med. Biol. 1996;404:415–428.
Mimaki Y, Kuroda M, Ido A, Kameyama A, Yokusuka A, Sashida Y. Steroidal saponin from the aerial parts of Dracaena draco and their cytostatic activity on HL 60 cells. Phytochemistry. 1999;50(5):805–813.
Yokosuka A, Mimaki Y, Sashida Y. Steroidal saponin from Dracaena draco. J. Nat. Prod. 2000;63(90):1239–1243.
Nong X. Hemostatic effect of Dracaena cochinensis (Lour) S.C. Chen. Zhongguo Zhong Yao ZA Zhi. 1997;22(4):240–243.
Bruck M. Dragon’s blood. A glance into the history of pharmacognosy. Bull. Soc. Sci. Med. 1999;1:96–101.
Ichikawa K, Kitaoka M, Taki M, Takaish IS, Iijima Y, Boriboon M, Akiyama T. Retrodihydrochalcones and homoisoflanes isolated from Thai medicinal plant Dracaena loureiri and their estrogen against activity. Planta Med. 1997;63(6): 648–656.
Junaid A, Mujib A, Sharma MP. Cell and tissue culture of Dracaena sanderiana Sander ex Mast. – A review. Hamdard Med. 2010;52(4):31–36.
Venkataramaiah V, Prasad SV, Rajeswara RG, Swamy PM. Levels of phenolic acids in Pterocarpus santalinus L. Indian J. Exp. Biol. 1980;18:887–889.
Bhattacharjee SK. Advances in ornamental horticulture. Pointer, Jaipur. 2006;6:2065. ISBN: 81-7132-432-0.
Parveen S, Shahzad A. Encapsulation of nodal segments of Cassia angustifolia Vahl. for short-term storage and germplasm exchange. Acta Physiol. Plant. 2014;36(3):635–640.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473-497.
Polanco MC, Pelaez MI, Ruiz ML. Plant cell, tissue and organ culture. 1988;175-182.
Nagata T, Takebe I. Plating of isolated tobacco mesophyll protoplasts on agar medium. 1971;99:12-20.
Redenbaugh K, Paasch BD, Nichol JW, Kossler ME, Viss PR, Walker KA. Somatic seeds: Encapsulation of asexual plant embryos. Nat. Biotechnol. 1986;4:797–801.
Anuradha T, Kumar KK, Balasubramanian P. Cyclic somatic embryogenesis of elite Indian cassava variety H-226. Ind. J. Biotechnol. 2015;14:559-565.
Nor-Asmah H, Nor-Hasnida H, NashatulZaimah NA, Noraliza A, NadiahSalmi N. Synthetic seed technology for encapsulation and regrowth of in vitro-derived Acacia hyrid shoot and axillary buds. African Journal of Biotechnology. 2011;10(40):7820-7824.
Sharma S, Shahzad A. Encapsulation technology for short-term storage and conservation of a woody climber, Decalepis hamiltonii Wight and Arn. Plant Cell Tissue Organ Cult. 2012;111:191–198.
Pattnaik S, Chand PK. Morphogenic response of the alginate encapsulated axillary buds from in vitro shoot cultures of six mulberries. Plant Cell Tissue Organ Cult. 2000;60:177–185.