Effect of Storage Temperatures on Phenotypic and Gene Expression of Maize (Zea mays L.) Genotypes

Main Article Content

Odunayo Joseph Olawuyi
Onyinye Mirian Chikeluba
Akinlolu Olalekan Akanmu

Abstract

Introduction: Maize is an important cereal grown globally across wide range of altitude and latitude. Temperature is one of the factors that affect the viability of maize under storage conditions.

Aim: This study, therefore, assessed the effect of different temperature storage levels on the morphological characters and molecular variability of maize genotypes.

Materials and Methods: The seeds of maize genotypes: TZLCOMP4C3, EVDT-W200STRCO, POP66SR/ACR94, POOL18SR QPM, TZM 132, TZM 1291, EVDT- Y2008 STR and TZM 1326 obtained from the National Centre for Genetic Resources and Biotechnology (NACGRAB) Ibadan were stored at different temperature conditions of -80°C, -20°C, 5°C, 50°C, and 25°C for 6 hours. The stored maize was sown in perforated polythene bags containing 7 kg of soil and replicated thrice in a complete randomized design. The molecular variability was also investigated on the maize genotypes stored under the varying temperatures. The effect of storage temperature was significantly higher at 50°C for all growth characters but was not significant at -80°C.

Results: The genotypic effect on the growth characters was significantly (p< 0.05) higher in TZM 132 with plant height (99.09 cm), leaf length (46.54 cm), leaf width (3.05 cm), number of leaves (6.25) and stem length (50.12 cm). The contribution of principal component axis (PCA) showed that PC 1 had the height variation with a proportion of 47.17% and eigen value of 2.83 across the growth characters. Molecular evaluation showed that EVDT-W200STRCO had the highest DNA concentration of 4885.7 ng/μl at storage temperature of 25°C, while EVDT-W200STCO at -20°C recorded the least DNA concentration of 26.60 ng/μl. The highest DNA concentration across the maize varieties were recorded at -20°C (POOL18SR QPM, TZM 132 and TZM 1326), 5°C (TZLCOMP4C3), 25°C (EVDT-W200STRCO and EVDT- Y2008 STR) and 50°C (POP66SR/ACR94 and TZM 1291). OPB 10 had the highest allelic no, gene diversity and polymorphic information content of 15, 0.97 and 97.0% respectively. The genetic distance matrix established relationship among the stored maize genotypes.

Conclusion: Maize seeds can therefore be stored at temperature range of -20°C to 50°C depending on the variety, without losing its viability and molecular constituents.

Keywords:
Maize, phenotypic characters, RAPD, temperature, storage levels.

Article Details

How to Cite
Olawuyi, O. J., Chikeluba, O. M., & Akanmu, A. O. (2020). Effect of Storage Temperatures on Phenotypic and Gene Expression of Maize (Zea mays L.) Genotypes. Biotechnology Journal International, 24(1), 38-52. https://doi.org/10.9734/bji/2020/v24i130096
Section
Original Research Article

References

Olakojo SA, Kogbe JO, Olajide V, Dohniell A. Host parasite relationship of Striga asiatica and maize (Zea mays) under varied moistuer level and nitrogen sources. Nigerian Journal of Weed Science. 2001;14:41-46.

Olakojo SA, Olaoye G. Response of maize (Zea mays L) to nitrogen fertilizer rate formulations under Striga lutea (LOUR) artificial infestation. Tropical and Subtropical Agroecosystems. 2007;7:21–28.

Olawuyi OJ, Odebode AC, Olakojo SA, Popoola OO, Akanmu AO, Izenegu JO. Host-pathogen interaction of maize (Zea mays L.) and Aspergillus niger as influenced by Arbuscular Mycorrhizal Fungi (Glomus deserticola). Archives of Agronomy and Soil Science. 2014;60(11): 1577–1591.

Olawuyi OJ, Odebode AC, Olakojo SA. Genotypes x concentrations x treatment concentration interaction and character association of maize (Zea mays L) under arbuscular mycorrhiza fungi and Striga lutea Lour. In Proceedings of 37th Annual Conference of the Genetics Society of Nigeria. (GSN), Lafia, 20-24 October. 2013;210-219.

FAO. The status of food production in Zimbabwe. Global early warming unit, ministry of lands and agriculture, Harare; 2003.
Available:http://www.fao.org
(Accessed: 09, 2015)

Mark C. Christian Science Monitor; 2008.
(Retrieved: October 2017)

Krueger K, Goggi AS, Mullen RE, Mallarino AP. Phosphorus and potassium fertilization do not affect soybean storability. Agronomy Journal. 2012;104:405–414.

Barton LV. Effect of moisture fluctuation on the viability of seed in storage. Contributions of the Boyce Thompson Institute. 1943;13:35-45.

Vertucci CW, Roos EE. Theoretical basis of protocols for seed storage. Plant Physiology. 1990;94:1019-1023.

Vertucci CW, Roos EE. Theoretical basis of protocols for seed storage II. The influence of temperature on optimal moisture levels. Seed Science Research. 1993;3:201-213.

Delouche JC. Precepts of seed storage. Proceedings of the Mississippi State Seed Processors Short Course. 1973;93-122.

Bewley JD, Black M. Seeds: Physiology of development and germination. 2nd Edition. Plenum Press, New York. 1994;423.

Joshi SP, Prabhakar K, Ranjekar PK, Gupta VS. Molecular markers in plant genome analysis. 2011;1-19.

Matsuoka Y, Vigouroux Y, Goodman MM, Sanchez JG, Buckler E, Doebley J. A single domestication for maize shown by multilocus microsatellite genotyping. Proceedings of the National Academy of Science. 2002;99:6080-6084.

Farooq S, Arif M, Iqbal N. Utilization of RAPD markers for identification of cultivated and wild rice species. Pakistan Journal of Botany. 1995;27:127-138.

Dellaporta SL, Wood J, Hicks JB. A plant DNA minipreparation: Version II. Plant Molecular Biology Rep. 1983;1:19–21.

SAS Institute Inc.. SAS 9.1 for Windows. SAS Institute Inc. Cary, NC; 2003.

Bano S, Asalam M, Saleem M, Basara SM, Aziz K. Evaluation of maize accessions under low temperature stress at early growth stages. Journal of Animal and Plant Sciences. 2015;25(2):392-400.

Saleem UR, Muhammad A, Khadim H, Shahid H, Tanweer M, Abdul R, Rana AI. Evaluation of maize hybrids for tolerance to high temperature strees in Central Punjab. American Journal of Bio-engineering and Biotechnology. 2013;1(1): 30-36.

Steven J, Brandner C, Salvucci M. Sensitivity of photosynthesis in C4 maize plant to heat stress. Plant Physiology. 2002;129:1773-1780.

Ihsan H, Khalil IH, Rehman H, Iqbal M. Genotypic variability for morphological traits among exotic maize hybrids. Sarhad Journal of Agriculture. 2005;21(4):599-602.

Abayi IK, Ojo AA, Kalu BA, Adeyemo MO. Genetic variability, heritability and genetic variance in S1 progenies of extra-early and early maize (Zea mays L.) population. Journal Sustainable Agriculture Environment. 2004;6:179-184.

Mubvuma MT, Mapaula S, Mashonjowa E. Effect of storage temperature and duration on germination of moringa seeds (Monringa oleifera). Greeves Journal of Agricultural Sciences. 2013;3(5):427-432.

Morroco A, Lorenzoni C, Fracheboud Y. Chilling stress in maize. Maydica. 2005;50: 571-580.

Olawuyi OJ, Bello OB, Ntube CV, Akanmu AO. Progress from selection of maize cultivars’ response to drought in the derived savanna of Nigeria. Agrivita Journal of Agriculture Science. 2015;37(1): 8-17.

Golbashy M, Khavari KS, Ebrahimi M, Choucan R. Evaluation of drought tolerance of some corn (Zea mays L.) hybrids in Iran. African Journal of Agricultural Research. 2010;5(19):2714-2719.