Biotechnology Journal International 2020-02-23T08:41:32+00:00 Biotechnology Journal International Open Journal Systems <p style="text-align: justify;"><strong>Biotechnology Journal International (ISSN:&nbsp;2456-7051)</strong> publishes original research papers, review articles and short communications on all areas of Biotechnology including cell biology, genetics, microbiology, immunology, molecular biology, biochemistry, embryology,&nbsp; immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, pharmacogenomics, gene therapy, plant, animal, microbial and environmental biotechnology.&nbsp;The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> Isolation, Screening and Identification of Cellulolytic Bacteria from Soil 2020-02-04T04:55:01+00:00 Pratibha Maravi Anil Kumar <p><strong>Background: </strong>Cellulose is the most abundant carbohydrate on earth and is considered as a good candidate for production of second generation biofuel (ethanol) and many other products of routine use. For degradation, cellulases are used which are mostly secreted by microbes such as fungi. Cellulases also play an important role in senescence of plants and in host-parasite relationship for invading the plant cell wall. However, comparatively lesser studies have been carried out on cellulase producing bacteria. Therefore, present study was aimed to isolate cellulase (Endo-β-1,4-D-glucanase; EC. from bacterial sources.</p> <p><strong>Methodology:</strong> To isolate thermophilic/ mesophilic cellulase producing bacteria, soil samples were collected from wood furnishing area and agricultural farm around Indore. Besides, soil sample was also collected from the vicinity of Amlai Paper Mill in Budhar district, Madhya Pradesh. These soil samples after suitable dilutions were streaked on different nutrients agar petri-dishes having carboxymethyl cellulose (CMC) as an inducer. After screening, four colonies were isolated capable of producing good amount of cellulase. Screening was done using Congo red staining and confirmation was done after growth of the bacteria in liquid nutrient medium having CMC. These colonies individually were grown in suitable nutrient media having CMC as an inducer and enzyme activity was determined in the nutrient media after harvesting bacterial cells by centrifugation.</p> <p><strong>Results: </strong>The highest enzyme producing bacteria were identified as <em>Bacillus lichenoformis</em> and <em>Ochrobactrum anthropi</em> after biochemical analyses, 16S rRNA sequencing and subsequently phylogenetic tree analysis.</p> 2020-01-31T00:00:00+00:00 ##submission.copyrightStatement## Genotoxicity Assessment of Potassium Bromate by Means of DNA Image Analysis on the Root Tip Nuclei of Allium sativum L. 2020-02-23T08:41:32+00:00 Hoda, A. Khatab Nagat, S. Elhaddad Samia Eissa <p><em>Allium sativum</em> assay was widely used to assess the compound's cytotoxicity and genotoxicity on plants and animals. Only few studies analyzed the genotoxic effect of potassium bromated (KBrO<sub>3</sub>) on the DNA content of plant cells. DNA content assay is an efficient test for the measure of chromosomal DNA damages. Based on this approach the effect of KBrO<sub>3</sub> on DNA content change was investigated in root tip nuclei of <em>A. sativum. </em>Different concentrations of food additives KBrO<sub>3</sub>. 3 g/l, 5 g/l 7 g/l and 9 g/l % were prepared and treatments were given. <em>A. sativum </em>root were incubated for 2, 6 and 24 hours and DNA image analysis of root tip nuclei was performed. The analysis was based on the measurement of the Mean Optical Density (MOD) which represents the cellular DNA content. The results showed that the KBrO<sub>3</sub> significantly decreased of DNA content compared to the control at all concentrations and treatment periods in dose-dependent manner. The present study suggests that extensive use of food additives should be banned due to genotoxic effect on living cells. Therefore, there is an urgent need to evaluate potential mutagenic effects of KBrO<sub>3</sub> on human.</p> 2020-02-05T00:00:00+00:00 ##submission.copyrightStatement## Analysis of Water Stress in Different Varieties of Maize (Zea mays L.) at the Early Seedling Stage 2020-02-23T08:41:31+00:00 Kiran Ramesh Pawar Sopan Ganpatrao Wagh Pravin Prakash Sonune Sakshi Raju Solunke Shubham Babanrao Solanke Sandeep G. Rathod Sanjay N. Harke <p>Maize (<em>Zea mays</em> L.) is a widely grown crop with a high rate of photosynthetic activity due to its C4 pathway leading to higher yields of grain and a potential for biomass. It is predominantly cross-pollinated crop, a feature that has contributed to its wide morphological variability and geographical adaptability. Abiotic stress such as drought stress negatively affects plant growth and development. The present study was designed to investigate the effects of drought stress on the morphological and biochemical content of seven different maize varieties. Here we have tested 7 different varieties of maize from the Marathwada region of Maharshtra, India. One week old plants have been affected by drought stress. After one week of drought stress, the plants were subjected to various morphometric and biochemical tests. The results showed that water stress treatment significantly affects root length, shoot length and fresh biomass of seven different varieties, <em>i.e</em>. TMMH 806, NMH 1008, DELTA 10V30, INDAM 1122, SRIKAR 3555, DKC 9141, SGA. Drought stress also had a negative impact on chlorophyll and proline content. Result further exhibited that based on drought tolerance index, variety SRIKAR 3555 could be ranked as drought tolerant and NMH 1008 as drought-sensitive, while remaining cultivator ranked as drought intermediates.</p> 2020-02-13T00:00:00+00:00 ##submission.copyrightStatement##