Biotechnology Journal International http://journalbji.com/index.php/BJI <p style="text-align: justify;"><strong>Biotechnology Journal International (ISSN:&nbsp;2456-7051)</strong> publishes original research papers, review articles and short communications on all areas of Biotechnology including cell biology, genetics, microbiology, immunology, molecular biology, biochemistry, embryology,&nbsp; immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, pharmacogenomics, gene therapy, plant, animal, microbial and environmental biotechnology.&nbsp;The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Biotechnology Journal International 2456-7051 Nano-Bio Challenge: New Approaches in Agricultural Production http://journalbji.com/index.php/BJI/article/view/30103 <p>Despite climate change scenarios, new research on improving yield and quality in agricultural production is extremely important. The use of nanomaterials and plant-growth-promoting bacteria (PGPB) has been of interest to researchers in recent years. In the future, the creation of new nano-biotechnological products by using nanomaterials together with bacteria will be more advantageous than conventional methods. Thus, the number of fertilizers applied on farmland will be reduced and maximum efficiency will be achieved with minimum input. The number of chemical inputs applied to agricultural areas will be reduced and effective protection against various plant stress factors will be provided. By producing Nano-bio-active. It is expected to increase mineral availability in farmland according to chemical formulations. The results obtained are certain to provide an effective benefit to the agricultural area and nature. Also, nano-biotechnological methods with new research potential are important for serving scientific researchers.</p> Kağan Tolga Cinisli Ela Akin Neslihan Dikbaş ##submission.copyrightStatement## 2020-05-14 2020-05-14 15 18 10.9734/bji/2020/v24i330103 Comparative Physiological, Biochemical and Transcript Response to Drought in Sorghum Genotypes http://journalbji.com/index.php/BJI/article/view/30102 <p>Sorghum [<em>Sorghum bicolor</em> (L.) Moench] is considered as an important staple crop in the tropical regions. However, the productivity of this useful crop is hindered by drought which contributes to significant yield reduction. The present study aimed to decipher the effects of drought stress on physiological, biochemical and gene expression changes in sorghum genotypes and to ascertain the differences in their response to drought stress. To achieve these objectives, six sorghum genotypes were grown in pot culture in a greenhouse, in a randomized complete block design and exposed to water stress treatment for 10 days. From the study, drought stress caused a significant (<em>P </em>&lt; .05) reduction in plant height, leaf water and chlorophyll contents while the proline, malondialdehyde (MDA), soluble sugar, electrolyte leakage (EL), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and antioxidant enzymes activity increased significantly (<em>P </em>&lt; .05) and differentially in all sorghum genotypes. Among the genotypes investigated, PI 585456 showed enhanced performance and was considered as the most tolerant to drought in relation to plant growth and water relation, membrane status, photosynthetic activity, ROS and osmolytes accumulation and antioxidant enzymes activity. Furthermore, the transcript expression analyses of different categories of drought-responsive genes, viz; antioxidant-related, osmolytes biosynthesis-related, dehydrin-related, photosystem-related and transcription-related were differentially upregulated in sorghum genotypes investigated. The results revealed the differences in metabolic response to drought among the genotypes, which accentuated the physiological, biochemical and molecular mechanism related to a specific response that may play a vital role in drought tolerance in sorghum.</p> Joseph Noble Amoah Daniel Antwi-Berko ##submission.copyrightStatement## 2020-04-22 2020-04-22 1 14 10.9734/bji/2020/v24i330102 Isolation of High Lignolytic Bacteria from Termites’s Gut as Potential Booster in for Enhanced Biogas Production http://journalbji.com/index.php/BJI/article/view/30104 <p>Bacteria strain capable of degrading lignin, cellulose and hemicellulose were isolated from wood Feeding termite gut using spread plate technique. The 16S rRNA gene sequencing methodology was adopted in the identification of the isolate. The isolate’s <em>Morganella morganii </em>(strain S4L2C (MH745964) were found to have a high lignin degradation potential. The organism was able to reduce the lignin content of rice straw from 17.43% to 7.29% after 30 days of pretreatment with 53.27% reduction of the lignin content. This study revealed that termite’s gut bacteria are Potential sources of lignocellulose degrading bacteria for the biological conversion of biomass to biogas production.</p> U. S. Anukam J. N. Ogbulie C. Akujuobi W. Braide ##submission.copyrightStatement## 2020-05-18 2020-05-18 19 23 10.9734/bji/2020/v24i330104 Identification and Expression Analysis of Stress Responsive Genes in Lentil (Lens culinaris) http://journalbji.com/index.php/BJI/article/view/30105 <p>Plants during their growth, experience periodic stress conditions both abiotic (adverse environmental conditions) as well as biotic (infection by pathogens). They appear to respond to these adverse conditions by modulating the expression of many genes. One of the pronounced effects of stress on plant is the enhanced synthesis of a set of proteins-termed ' stress proteins'. Lentil contains asset of genes/proteins which helps this crop to overcome abiotic stresses. In the present study, HSP70 (Heat Shock Protein), LEA (Late Embryogenesis Abundant) and Aldolase genes were identified and cloned in pTZ57RT vector followed by sequencing. Expression analysis was done through Q-PCR which was assessed by using cDNA from all the heat, drought and salinity stressed and unstressed lentil cotyledons. The highest level of transcript of HSP70 was realized upon exposure to heat at 45°C for 3 hour followed by at 45°C for 2 hour and lowest at 40°C for 1hour. LEA gene was identified under drought and salinity stress and highest transcript was at 20% PEG for 3 hour (drought stress) and in salinity stress highest transcript was at 150 mm for 6 hour.&nbsp; For Aldolase gene highest transcript was recorded after 3, 6 and 12 hr at 100 mM, 150 mM, 200 mM of salinity stress respectively.&nbsp; From these studies it can be concluded that heat shock protein gene, LEA, and aldolase present in lentil which can be exploited in overcoming the abiotic stresses for obtaining the higher productivity in crop plants through genetic engineering.</p> Annu Yadav Himanshi . Shruti . Jitender Singh Pankaj Kumar Shivani Khanna Anil Sirohi ##submission.copyrightStatement## 2020-05-18 2020-05-18 24 34 10.9734/bji/2020/v24i330105