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Euphorbia fusiformis is a rare medicinal plant. The genus Euphorbia belongs to the family Euphorbiaceae. E. fusiformis is commercially useful for production of latex and has many medicinal values. Based on the importance of the plant, it is selected for plant tissue culture work. The present research work is to establish somatic embryogenesis from explants of leaf. It is first attempt of the regeneration studies in E. fusiformis through somatic embryogenesis. For plantlet regeneration studies MS medium supplemented with α- Naphthlene Acetic Acid (NAA) 2.0 mg/L and 2, 4- Dichloro Phenoxy acetic acid ( 2,4- D ) 2.0 mg/L. were used.