Viability of Hydrocarbon-degrading Bacterial Consortium Immobilized on Different Carriers

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Victor Ezebuiro
Ipeghan Jonathan Otaraku
Boma Oruwari
Gideon Chijioke Okpokwasili


Aim: Viability of hydrocarbon-degrading bacterial consortium immobilized on different carriers was studied.

Methodology: Hydrocarbon-degrading bacteria were isolated from crude oil contaminated sites in Gio and K-Dera, Rivers State, Nigeria using enrichment method. Proximate analyses were carried out on the best carrier materials. Immobilization was by direct adsorption of the isolates onto the carrier materials and viability was determined by plate count method. The carrier materials tested included soya bran, sugarcane bagasse, corn cob, brown saw dust, white saw dust, cassava peel and red mud (bentonite).

Results: The bacterial isolates demonstrated varied degradation capacity. The best carrier material was saw dust (103.6% survival) and corn cob (103.6% survival) followed by soya bran (94.4% survival rate) and cassava peel (94.4% survival rate). The saw dust had moisture content, 5.92%; ash content, 7.49%; crude protein, 2.2%; volatile matter, 74.28; and fixed carbon, 12.34%; whereas, the percentage chemical composition observed for soya bran were 10.11, 4.08, 5.22, 42.61, 18.37 and 8.89 for moisture content, ash content, crude fibre, crude protein, crude fat and carbohydrate, respectively. There was significant difference (p=0.05) between viability rate observed with the different carrier materials.

Conclusion: This study showed that the agro-wastes used in this study can effectively enhance the viability of hydrocarbon-utilizing bacterial. The result is significant as it shows the possibility of using these carrier materials for bioremediation of hydrocarbon contaminated media.

Carrier materials, hydrocarbon-utilizing bacteria, immobilization, viability, saw dust and soya bran

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How to Cite
Ezebuiro, V., Otaraku, I. J., Oruwari, B., & Okpokwasili, G. C. (2019). Viability of Hydrocarbon-degrading Bacterial Consortium Immobilized on Different Carriers. Biotechnology Journal International, 23(4), 1-9.
Original Research Article


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