Isolation of High Lignolytic Bacteria from Termites’s Gut as Potential Booster in for Enhanced Biogas Production

Main Article Content

U. S. Anukam
J. N. Ogbulie
C. Akujuobi
W. Braide


Bacteria strain capable of degrading lignin, cellulose and hemicellulose were isolated from wood Feeding termite gut using spread plate technique. The 16S rRNA gene sequencing methodology was adopted in the identification of the isolate. The isolate’s Morganella morganii (strain S4L2C (MH745964) were found to have a high lignin degradation potential. The organism was able to reduce the lignin content of rice straw from 17.43% to 7.29% after 30 days of pretreatment with 53.27% reduction of the lignin content. This study revealed that termite’s gut bacteria are Potential sources of lignocellulose degrading bacteria for the biological conversion of biomass to biogas production.

Biodelignification, termites gut, gene sequence, biogas

Article Details

How to Cite
Anukam, U. S., Ogbulie, J. N., Akujuobi, C., & Braide, W. (2020). Isolation of High Lignolytic Bacteria from Termites’s Gut as Potential Booster in for Enhanced Biogas Production. Biotechnology Journal International, 24(3), 19-23.
Original Research Article


Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 1985;39:783-791

Moiser N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol. 2005;96:673-686.

Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN, editor, Mammalian Protein Metabolism, Academic Press, New York. 1969;21-132.

Gao C, Xiao W, Ji G, Zhang Y, Cao Y, Han L. Regularity and mechanism of wheat straw properties change in ball milling pro-cess at cellular scale. Bioresour Technol. 2017;241:214–219.

Chandra R, Raj A, Purohit HJ, Kapley A . Characterisation and optimization of three potential aerobic bacterial strains for kraft lignin degradation from pulp paper waste Chemosphere. 2007;67:839–846.

Saitou N, Nei M. The neighbor- joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 1987;4: 406-425.

Zeng Y, Yang X, Yu H, Zhang X, Ma F. The delignification effects of white-rot fungal pretreatment on thermal characteristics of moso bamboo. Bioresour Technol. 2012;114:437–442.

Shi Y, Yan X, Li Q, Wang X, Xie S, Chai L, Yuan J. Directed bioconversion of Kraft lignin to polyhydroxyalkanoate by Cupri-avidusbasilensis B-8 without any pretreatment. Process Biochem. 2017;52: 238–242.