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Plants during their growth, experience periodic stress conditions both abiotic (adverse environmental conditions) as well as biotic (infection by pathogens). They appear to respond to these adverse conditions by modulating the expression of many genes. One of the pronounced effects of stress on plant is the enhanced synthesis of a set of proteins-termed ' stress proteins'. Lentil contains asset of genes/proteins which helps this crop to overcome abiotic stresses. In the present study, HSP70 (Heat Shock Protein), LEA (Late Embryogenesis Abundant) and Aldolase genes were identified and cloned in pTZ57RT vector followed by sequencing. Expression analysis was done through Q-PCR which was assessed by using cDNA from all the heat, drought and salinity stressed and unstressed lentil cotyledons. The highest level of transcript of HSP70 was realized upon exposure to heat at 45°C for 3 hour followed by at 45°C for 2 hour and lowest at 40°C for 1hour. LEA gene was identified under drought and salinity stress and highest transcript was at 20% PEG for 3 hour (drought stress) and in salinity stress highest transcript was at 150 mm for 6 hour. For Aldolase gene highest transcript was recorded after 3, 6 and 12 hr at 100 mM, 150 mM, 200 mM of salinity stress respectively. From these studies it can be concluded that heat shock protein gene, LEA, and aldolase present in lentil which can be exploited in overcoming the abiotic stresses for obtaining the higher productivity in crop plants through genetic engineering.
Shao HB, Chu LY, Jaleel CA, Zhao CX Water-deficit stress-induced anatomical changes in higher plants. C. R. Biol. 2008; 331:215-225.
Abuqamar S, Luo H, Laluk K, Mickelbart, MV, Mengiste T. Crosstalk between biotic and abiotic stress responses in tomato is mediated by the AIM1 transcription factor. Plant J. 2009;58: 347–60.
Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, Yamaguchi-Shinozaki K, et al. Crosstalk between abiotic and biotic stress responses: A current view fromthe points of convergence in the stress signaling networks. Curr. Opin. Plant Biol, 2006;9:436–42.
Jakab G, Ton J, Flors V, Zimmerli L, Metraux JP, Mauch-Mani B. Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses. Pl. Physiol. 2005; 139:267–74.
Ton J, Jakab G, Toquin V, Flors V, Iavicoli A, Maeder MN, et al. Dissecting the beta-amino butyric acid-induced priming phenomenon in Arabidopsis. Pl. Cell. 2005;17:987–99
Haralampidis K, Milioni D, Rigas S, Hatzopoulos P. Combinatorial interaction of cis elements specifies the expression of the Arabidopsis AtHsp90-1 gene. Pl. Physiol. 2002;129:1138–49.
Volkov RA, Panchuk II, Schoffl F. Small heat shock proteins are differentially regulated during pollen development and following heat stress in tobacco. Plant Mol. Biol. 2005;57:487–502.
Reddy RK, Chaudhary S, Patil P, Krishna P. The 90kDaheat shock protein (hsp90) is expressed throughout Brassica napus seed development and germination. Pl. Sci. 1998;131:131–7.
Sun Y, MacRae TH. (Small heat shock proteins: molecular structure and chaperone function. Cell. Mol. Life Sci. 2005;62:2460–2476 Available:https://doi.org/10.1007/s00018-005-5190-4
Rutter WJ. (Evolution of aldolase. Fed Proc Am Soc Biol. 1964;23:1248–1257.
Lebherz HG, Leadbetter MM, Bradshaw RA. Isolation and characterization of cytosolic and chloroplastic forms of spinach leaf fructose diphosphate aldolase. J Biol Chem. 1984;259:1011–1017.
Fan W, Zhang Z, Zhang Y. (Cloning and molecular characterization of fructose-1,6- bisphosphate aldolase gene regulated by high-salinity and drought in Sesuvium portulacastrum. Pl. Cell Rep. 2009;28: 975–984.
Dita MA, Rispai N, Rubiales E, Prats D, Singh KB. Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes. Euphytica. 2006; 147:1–24.
FAOSTATS. Food and Agriculture Organization of the United Nations; 2018. Available:http://www.fao.org/faostat/en/.
Lamaoui M, Jemo M, Datla R, Bekkaoui F. Heat and drought stresses in crops and approaches for their mitigation. Front Chem. 2018;6:26.
Sarkar NK, Kim YK, Grover A. Coexpression network analysis associated with call of rice seedlings for encountering heat stress. Pl. Mol. Biol. 2014;84:125–143.
Cox KH, Goldberg RB. Analysis of plant gene expression. In plant molecular biology, a practical approach, C.H. Shaw, ed (Washington, DC: IRL Press). 1988;1-35.
Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 2013;3(3): 71‐85.
Thompson JD, Higgins DG, Gibson TJ Clustal W. improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Research. 1994; 22(22):4673–4680.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary Genetic analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. 2011;28:2731-2739
Pavli OI, Ghikas DV, Katsiotis A, Skaracis GN. Differential expression of heat shoc2k protein genes in sorghum (Sorghum bicolor L.) genotypes under heat stress. Aust. J. Crop Sci. 2011;5:511–515.
Kumar A, Nidhi, Prasad N, Sinha SK. Nutritional and antinutritional attributes of faba bean (Vicia faba L.) germplasms growing in Bihar, India. Physiol Mol Biol Plants. 2015; 21(1):159-162.
Zhao Y, Yun JF, Shi FM, Wang JJ, Yang QC, Chao YH. Molecular cloning and characterization of a group 3 LEA gene from Agropyron mongolicum Keng. Afr. J. Biotechnol. 2010;9(37):6040-6048.
Kumar RR, Goswami S, Sharma KS, Singh K, Gadpayle AK, Singh DS, Pathak H, Rai DR. Differential expression of heat shock protein and alteration in osmolyte accumulation under heat stress in wheat. J Plant Biochem Biot. 2013;22:16-22.
Zhang K, Ezemaduka NA, Wang Z, Hu H, Shi X, Liu C, Lu X, Fu X, Chang Z, Yin CC. A novel mechanism for small heat shock proteins to function as molecular chaperones. Sci. Rep. 2015;5:8811
Hu Wenhuo, Hu Guocheng, H Bin. Genome-wide survey and expression profiling of heat shock proteins and heat shock factors revealed overlapped and stress specific response under abiotic stresses in rice . Pl. Sci. 2009;176:583–590.
Wang X, Manning W, Feng Z, Zhu Y. Ground-level ozone in China: distribution and effects on crop yields. Environ. Pollut. 2007;147:394–400.
Ramezani A, Naizi A, Moghadam A, Babgohari MZ. Quantitative expression analysis of TaSOS1 and TaSOS4 genes in cultivated and wild wheat plants under salt stress. Mol Biotechnol. 2012;53(2):189-197.
Yang E, Yi S, Bai F, Niu D, Zhong J, Wu Q, Chen S, Zhou R, Wang F. cloning, characterization and expression pattern analysis of a cytosolic copper/zinc superoxide dismutase (SACSD1) in a highly salt tolerant mangrove. Int J Mol Sci. 2015;17(1):4.