Phenotypic Characterization of Fungal Pathogens Associated with the Main Mycoses of Cashew (Anacardium occidentale L.)

Komi Lucien Domedjui

Laboratory of Botany and Plant Ecology, University of Lome, 01, P.O.Box- 1515, Lome, Togo.

Kodjo Djidjolé Etse *

Forestry Research Laboratory, University of Lome, 01, P.O.Box- 1515, Lome, Togo.

Koffi Apeti Gbogbo

Laboratory of Botany and Plant Ecology, University of Lome, 01, P.O.Box- 1515, Lome, Togo.

Kossi Essotina Kpemoua

Crop Protection and Biosafety Laboratory, Togolese Institute of Agronomic Research (ITRA), P.O.Box- 1163, Lome, Togo.

Ekanao Tedihou

Crop Protection and Biosafety Laboratory, Togolese Institute of Agronomic Research (ITRA), P.O.Box- 1163, Lome, Togo.

Rassimwaï Pitekelabou

Crop Protection and Biosafety Laboratory, Togolese Institute of Agronomic Research (ITRA), P.O.Box- 1163, Lome, Togo.

Tchaou B. P. I. Bodjona

Crop Protection and Biosafety Laboratory, Togolese Institute of Agronomic Research (ITRA), P.O.Box- 1163, Lome, Togo.

N’pagyendou Lare

Crop Protection and Biosafety Laboratory, Togolese Institute of Agronomic Research (ITRA), P.O.Box- 1163, Lome, Togo.

Essowè Palanga

Crop Protection and Biosafety Laboratory, Togolese Institute of Agronomic Research (ITRA), P.O.Box- 1163, Lome, Togo.

Koffi Akpagana

Laboratory of Botany and Plant Ecology, University of Lome, 01, P.O.Box- 1515, Lome, Togo.

*Author to whom correspondence should be addressed.


Abstract

Aims: To study the diversity of fungal pathogens associated with cashew mycoses in Togo.

Study Design: This research project was initiated by the Mycology Research and Applications Unit of the Botany and Plant Ecology Laboratory (LBEV) in order to have adequate information on cashew mycoses in Togo.

Place and Duration of Study: Laboratory of Botany and Plant Ecology (LBEV) of the University of Lome (UL) and of Crop Protection and Biosafety Laboratory of Togolese Institute of Agronomic Research (ITRA), February to August 2020.

Methodology: A total of 148 symptomatic samples (leaves, buds, inflorescences, nuts, and apples) were collected from cashew trees in the East Mono prefecture of Togo. Malt-agar medium supplemented with chloramphenicol at 0.5 g/l was used for the isolation of fungal pathogens. The characterization of these fungal pathogens was carried out from the 7th day based on their macroscopic (texture, color, diameter of growth) and microscopic (hypha, spore, fruiting body) characters.

Results: This study revealed the presence of five mycoses in cashew orchards in the East Mono prefecture. These are leaf anthracnose, bud’s dieback, black rust, leaf yellowing, and powdery mildew. In total, 12 fungal genera were encountered and 14 species of fungal pathogens were identified on all the samples collected: Rhizopus sp., Penicillium sp., Mucor sp., Sporotrichum sp., Fusarium nivale, Fusarium moliniforme, Fusarium moliniforme var. subglutinans, Curvularia lunata, Curvularia geneculata, Alternaria tenuissima,  Alternaria brassicisola, Beltrania rhombica Penz., Thielavia coactilis Nicot, Helminthosporium avenae, Helminthosporium siccans, Phoma eupyrena, Aspergillus flavus, Aspergillus niger.

Conclusion: It would be of great interest to train cashew producers in the East Mono prefecture on the recognition of the symptoms of these mycoses and their management.

Keywords: Anacardium occidentale L., mycoses, fungal pathogens, phenotypic characterization, Togo


How to Cite

Domedjui, K. L., Etse, K. D., Gbogbo, K. A., Kpemoua, K. E., Tedihou, E., Pitekelabou, R., Bodjona, T. B. P. I., Lare, N., Palanga, E., & Akpagana, K. (2022). Phenotypic Characterization of Fungal Pathogens Associated with the Main Mycoses of Cashew (Anacardium occidentale L.). Biotechnology Journal International, 26(4), 1–10. https://doi.org/10.9734/bji/2022/v26i4652

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References

Trevisan MTS, Pfundstein B, Haubner R, Würtele G, Spiegelhalder B, Bartsch H, Owen RW. Characterization of alkyl phenols in cashew (Anacardium occidentale L.) products and assay of their antioxidant capacity. Food Chem. Toxicol. 2006;44(2):188- 197. Available:https://doi.org/10.1016/j.fct.2005.06.012

Faostat F. Agriculture organization corporate statistical database. Accessed 25 December; 2018.

Available:https://www.fao.org/faostat/en/#data

Delgado C, Couturier G. Principaux insectes nuisibles à l’anacardier en amazonie péruvienne. donnees préliminaires. Fruits. 2014;69(4):293-302.

Available:http://dx.doi.org/10.1051/fruits/2014018 French.

Houndahouan DET, Zannou A, Sikirou R, Adomou A, Zinsou V, Boukari S, N’djolossè K. Les pertes economiques dues a L’anthracnose de L’anacardier au benin. Eur. Sci. J. ESJ. 2018;14(15):127.

Available:https://doi.org/10.19044/esj.2018.v14n15p127. French.

Sijaona MER, Clewer A, Maddison A, Mansfield JW. Comparative analysis of powdery mildew development on leaves, seedlings and flower panicles of different genotypes of cashew. Plant Pathol. 2001;50(2):234-243.

Available:https://doi.org/10.1046/j.1365-3059.2001.00544.x

Sijaona MER, Shomari SH. The powdery mildew disease of cashew in Tanzania. TARO Newsl. 1987;2:4-5.

Annual cashew research report-ACRR. Chapter 3 Pathology Report. Naliendele Agricultural Research Institute, Mtwara, Tanzania. 2006;98-99.

Majune DJ, Masawe PA, Mbega ER. Status and management of cashew disease in Tanzania. Int. J. Environ. Agric. Biotechnol. 2018;3(5):1590-1597.

Available:http://dx.doi.org/10.22161/ijeab/3.5.4

Wonni I, Sereme D, Ouedraogo I, Kassankagno AI, Dao I, Ouedraogo L, Nacro S. Diseases of cashew nut plants (Anacardium Occidentale L.) in Burkina Faso. Adv Plants Agric Res. 2017;6(3): 78-83.

Available:https://doi.org/10.15406/apar.2017.06.00216

Assenga BB, Masawe PA, Tarimo TM, Kapinga F, Mbega ER. Status of sucking insect pests in cashew growing locations of south and central zones, Tanzania. International Journal of Biosciences. 2020; 16(4):34-45.

Available:https://doi.org/10.12692/ijb/16.4.34-45

Dufresne P, Guy SG. Identification des champignons d’importance médicale. Inst. Natl. Santé Publique Qué.

Accessed 25 july 2020. 2021;1-64.

Available:https://www.inspq.qc.ca/sites/default/files/lspq/identification_champignons_importance_medicale.pdf . French.

Domsch KH, Gams W, Anderson TH. Compendium of soil fungi. Academic Press (London) Ltd. 1980;1.

Botton B, Breton A, Fevre M, Gauthier S, Guy P, Larpent J, Reymond P, Sanglier J, Vayssier Y, Veau P. Moisissures utiles et nuisibles, importance industrielle. Masson, Paris, Milan, Barcelone. French; 1990.

Champion R. Identifier les champignons transmis par les semences. INRA, Paris. French; 1997.

Watanabe T. Pictorial atlas of soil and seed fungi: Morphologies of cultured fungi and key to species. CRC press; 2010.

Domedjui KL. Inventaire des pathologies fongiques dans les vergers d’anacardiers (Anacardium occidentale L.) de la préfecture d’Est-mono et essai de culture in vitro de la plante. Mémoire de Master, Université de Lomé,Togo. French. 2020;1-64.

Freire FCO, Cardoso JE, Dos Santos AA, Viana FMP. Diseases of cashew nut plants (Anacardium occidentale L.) in Brazil. Crop Prot. 2002;21(6):489-494.

Available:https://doi.org/10.1016/S0261-2194(01)00138-7

Lopes LF, Cruz AF, Barreto ML de A, Vasconcelos TMM de, Blum LEB. Post-harvest treatment with Ca-phosphite reduces anthracnose without altering papaya fruit quality. J. Hortic. Sci. Biotechnol. 2018;93:272-278.

Available:https://doi.org/10.1080/14620316.2017.1361342

Dooh JPN, Asta CBD, Djile B, Tchoupou DBT, Heu A, Mboussi SB, Kuate WNT and Ambang Z. Major fungi diseases of cashew trees (Anacardium occidentale L.) in Cameroon. Journal of Agricultural Science. 2021:13(3).

DOI:10.5539/jas.v13n3p124

Sijaona MER, Reeder RH, Waller JM. Cashew leaf and nut blight-a new disease of cashew in Tanzania caused by Cryptosporiopsis spp. Plant Pathol. 2006; 55(576).

Available:https://doi.org/10.1111/j.1365-3059.2006.01365.x

Menge D, Makobe M, Agboton B, Shomari S, Tiedemann A. Biology and infection mechanisms of cryptosporiopsis spp fungus causing blight disease on cashew (Anacardium Occidentale L.). J. Plant Sci. 2014;2(6):266-275.

Available:https://doi.org/10.11648/j.jps.20140206.12

Menge D, Makobe M, Shomari S. Effect of environmental conditions on the growth of Cryptosporiopsis spp. causing leaf and nut blight on cashew (Anacardium occidentale Linn.). J. Yeast Fungal Res. 2013;4:12-20.

Available:https://doi.org/10.5897/JYFR12.006

Aihounton GB. Lutte contre les maladies fongiques dans les plantations d’anacardiers; 2017.

Accessed 28 july 2019.

Available:http://www.runetwork.org/html/fr/articles/12513.html French.

Lissava I. Plusieurs taches jaunâtres sur les feuilles d’anacardier. 2017. Accessed 28 july 2019.

Available:http://www.runetwork.org/html/fr/articles/12234.html French.

Kone D, Camara B, Abo K, Ouali-N’goran M, Cherif M, Robert ND, Sibirina S. Maladies et insectes ravageurs de l’anacardier. 2015.

Accessed 13 september 2019

Available:https://www.researchgate.net/publication/323880029. French.

Banito A, Kpemoua EK, Dayiwo RK, Tedihou E, Sikirou R. Inventaire des maladies de l’anacardier (Anacardium occidentale L.) dans la préfecture de Tchamba au Togo. Int. J. Biol. Chem. Sci. 2021;15(6):2514-2525.

Available:https://doi.org/10.4314/ijbcs.v15i6.21. French.

Afouda LCA, Zinsou V, Balogoun RK, Onzo A, Ahohuendo BC. Inventaire des agents pathogènes de l’anacardier (Anacardium occidentale L.) au Bénin. Bull. Rech. Agron. Bénin. 2013;73:13-19. French.

N’Guettia MY, Diallo HA, Kouassi N, Coulibaly F. Diversité morphologique et pathogénique des souches de Colletotrichum sp. responsables de l’anthracnose de la mangue en Côte d’Ivoire. J Anim Plant Sci. French. 2013;18 (3):2775-2784.

Morelet M. Le diagnostic des maladies fongiques en forêt. Rev. For. Fr; French ; 1988.

Nambiar KKN. Controlling cashew diseases [India]. Indian Farming; 1978.

Kiffer E, Morelet M. Les deutéromycètes: Classification et clés d’identification générique. Editions Quae, Paris. French; 1997.

Wang XW, Bai FY, Bensch K, Meijer M, Sun BD, Han YF, Crous PW, Samson RA, Yang FY, Houbraken J. Phylogenetic re-evaluation of Thielavia with the introduction of a new family Podosporaceae. Stud. Mycol. 2019;93(1):155-252.

Available:https://doi.org/10.1016/j.simyco.2019.08.002

Hektoen L, Perkins CF. Refractory subcutaneous abscesses caused by Sporothrix schenckii. A new pathogenic fungus. J. Exp. Med. 1900;5(1):77-89.

Available:https://dx.doi.org/10.1084%2Fjem.5.1.77

Thibaut M, Ansel M. Discovery of Ascomycete characteristics in Sporothrix schenckii. Rev. Soc. Bras. Med. Trop. 1973;7(6):341-348.

Available:https://doi.org/10.1590/S0037-86821973000600003