Phytochemical Screening and Antimicrobial Activity of Sarcocephalus latifolius Smith Roots Extracts
Biotechnology Journal International, Volume 26, Issue 6,
Page 54-62
DOI:
10.9734/bji/2022/v26i6665
Abstract
Aims: This work aims to evaluate the antimicrobial activity of Sarcocephalus latifolius extracts.
Methodology: Thus, phytochemical screening was qualitatively accessed using colorations or precipitations methods. Ethanolic and aqueous extracts were used to evaluate the antimicrobial activity. The antimicrobial activity, using the diffusion method, was evaluated on eight strains including two reference strains (Streptococcus pneumoniae ATCC 49619 and Pseudomonas aeruginosa ATCC 27853) and six clinically isolated S. pneumoniae and P. aeruginosa strains. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined by the microdilution method.
Results: The phytochemical screening showed the presence of flavonoids, anthocyanins, mucilages, saponosides, C-heterosides and O-heterosides. Antimicrobial activity showed that the ethanolic extract with the lowest MIC (1.25 mg/ml) inhibited reference strains (S. pneumoniae ATCC 49619 and P. aeruginosa ATCC 27853) and clinical isolated S. pneumoniae and P. aeruginosa strains. The largest inhibition diameter (19± 1.33) was obtained with the ethanolic extract against clinical isolated Pseudomonas aeruginosa and (15.5± 1) against the reference one. The aqueous extract inhibited only reference strains.
Conclusions: The data of this study indicate that the extracts of S. latifolius present antimicrobial properties. This may justify its traditional use in the treatment of microbial infections.
- Phytochemical screening
- antimicrobial activity
- Sarcocephalus latifolius
- Benin
How to Cite
References
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