Effects of Aqueous Seed Extracts of Sphenostylis stenocarpa on the Reproductive Indices of Male Rats

E. F. Ogbuke

Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State, Nigeria.

D. E. Oboho *

Department of Animal and Environmental Biology, University of Uyo, Akwa Ibom State, Nigeria.

N. D. Ekpo

Department of Animal and Environmental Biology, University of Uyo, Akwa Ibom State, Nigeria.

E. O. Mbong

Department of Environmental Biology, Heritage Polytechnic Ikot Udota Eket, Nigeria.

A. U. Nelson

Department of Animal Science, Federal Polytechnic Ugep, Cross River State, Nigeria.

I. J. Udo

Department of Biological Sciences, Akwa Ibom State Polytechnic, Ikot Osurua, Nigeria.

N. I. Etukudo

Department of Botany and Ecological Studies, University of Uyo Akwa Ibom State, Nigeria.

V. C. Ejere

Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Effects of aqueous seed extracts of Sphenostylis stenocarpa on the reproductive indices of male rats were investigated. A total of 104 adult rats were used for the experiment, and were divided into 4 groups (group A – D) and replicated in triplicate. Group A served as the normal control, while groups B, C and D received three graded doses (800mg/kg, 1200mg/kg and 1600mg/kg) of the extracts, respectively, by oral intubation. The gonad characteristics, sperm parameters and hormonal analyses of the male rats were determined using standard procedures. These were ascertained prior to the commencement of treatment, and on weekly basis. Data were analyzed statistically using SPSS and R software at 95% confidence interval. An overall dose and time dependent showed significant differences in the mean weekly gonad characteristics of the male rats in the treatment groups when compared with the control. There was a significant reduction (p < 0.05) in the body weights of the male rats, but a significant increase (P < 0.05) in the testes weights, gonad somatic index, sperm count and sperm motility in the rats. The gonadal hormone testosterone, responded to the plant extracts, while follicle-stimulating and luteinizing hormones were largely undetected. There were significant increases in the testosterone levels of all the treated rats. Conclusively, aqueous seed extracts of Sphenostylis stenocarpa seems to possess ability to enhance reproductive health in male rats.

Keywords: Seed extract, Sphenostylis stenocarpa, Albino rats, reproductive indices


How to Cite

Ogbuke , E. F., Oboho , D. E., Ekpo , N. D., Mbong , E. O., Nelson , A. U., Udo , I. J., Etukudo , N. I., & Ejere , V. C. (2023). Effects of Aqueous Seed Extracts of Sphenostylis stenocarpa on the Reproductive Indices of Male Rats. Biotechnology Journal International, 27(1), 25–34. https://doi.org/10.9734/bji/2023/v27i1670

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