Production of Soft Bloomy Rind Cheese with Lactic Acid Bacteria Isolated from Cameroon’s Cow Milk

R. Tchikoua *

Department of Microbiology, Laboratory of Microbiology, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.

R. A. Nago Djoufack

Department of Microbiology, Laboratory of Microbiology, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.

R. B. Okamba Elouti

Department of Microbiology, Laboratory of Microbiology, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.

J. J. Essia Ngang

Department of Microbiology, Laboratory of Microbiology, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.

*Author to whom correspondence should be addressed.


The changing of the diversity of lactic acid bacteria (LAB) from one locality and another is one of the main reasons of the organoleptic and physicochemical differences encountered in the same class of cheese. The objective of this work was to evaluate the influence of LAB isolated from cow's milk produced locally in Cameroon on the organoleptic and physicochemical quality of soft cheese with bloomy rind. To this effect, 05 LAB (IS1, IS2, IS3, IS4 and IS5) were isolated to fresh milk and selected after macroscopic, microscopic and biochemical characterizations. After tests of acidification, fermentation type, compatibility between LAB and LAB concentration on milk coagulation, the combined IS1-IS4-IS5 and IS2-IS3-IS5 were retained to produce 02 soft cheeses with bloomy rind namely respectively FROCAM 145 and FROCAM 235. When used at concentrations of 3×107 CFU/mL, these combined LAB coagulate fresh renneted milk after 3 hours at 26°C. During the production of FROCAM 145, the combined IS1-IS4-IS5 resulted in a firm, moist curd and a pH reduction of 14 and 15% respectively after the milk maturation and ripening of FROCAM 145. This cheese recorded 100% mould recovery, with protein, lipid and calcium content of 6.7, 62.2 and 0.1% respectively. On the other hand, during the production of FROCAM 235, the combined IS2-IS3-IS5 resulted in a dry and crumbly curd. The pH recorded with this combined LAB after maturation of the milk and ripening of FROCAM 235 is 12 and 17% respectively. The FROCAM 235 recorded a low mould recovery estimated at 20% and a protein, lipid and calcium content of 7.8, 65.1 and 0.3% respectively.  From a microbiological point of view, FROCAM 145 and FROCAM 235 did not record any contamination by Listeria monocytogenes, Salmonella and Staphylococci. The sensory analysis shows that FROCAM 145 was more appreciated than FROCAM 235 with scores of 0.74 and 0.24 respectively. In view of these results, the IS1-IS4-IS5 combinations isolated from Cameroonian milks present a certain technological interest in the transformation of milk into cheese.

Keywords: Lactic acid bacteria, cheese, protein, lipid, calcium, sensory analysis

How to Cite

Tchikoua , R., Djoufack , R. A. N., Elouti , R. B. O., & Ngang , J. J. E. (2023). Production of Soft Bloomy Rind Cheese with Lactic Acid Bacteria Isolated from Cameroon’s Cow Milk. Biotechnology Journal International, 27(3), 8–21.


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