Evaluation of Saccharomyces cerevisiae Improved Strains Potential in the Bioethanol Production from Bagasse
Adegbehingbe Kehinde Tope *
Adekunle Ajasin University, Akungba-Akoko, Nigeria.
Adetuwo Olagunju Johnson
Adekunle Ajasin University, Akungba-Akoko, Nigeria.
Omodara Tolani Rachael
Ekiti State University, Ado-Ekiti, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Plant biomass can be utilized to produce bioethanol, because they are abundantly available in nature. The cost of ethanol production from lignocellulosic materials is relatively high with low yield. But this can be solved by strain improvement processes. This study is aimed at evaluate bioethanol production potential of improved strains of Saccharomyces cerevisiae developed through random mutagenesis. Bagasse was hydrolysed with 1% NaOH and 1.0M H2SO4 respectively for five days. The hydrolysed bagasse was saccharified using Aspergillus niger isolated from soil samples. Saccharomyces cerevisiae isolated from locally produced wines; sorghum (burukutu) oil palm wine (emu) and raphia palm wine (oguro) with the highest ethanol production (5.0g/ml) were used, and then treated with physical mutagen (ultraviolet light) and chemical mutagens (Acridine dye, Bromo acetaldehyde, dithiothreitol, Ketoconazole and Nitrous acid) respectively to develop mutant with high ethanol producing efficiency under varied operational parameters. Three mutant strains of Saccharomyces cerevisiae namely;- SUV, SCD and SCK produced higher volumes of ethanol (7.5 g/ml, 9.8 g/ml, 11.2 g/ml respectively). SCD and SCK were able to grow at 25% ethanol concentration indicating that they had higher ethanol tolerance ability than the other strains. The optimum temperature and pH for ethanol production by all the strains were 350C and 6.0 respectively. The improved strains of Saccharomyces cerevisiae developed through random mutation techniques had produced more ethanol from the bagasse than the wild-type.
Keywords: Biomass, mutants, tolerance, pH, temperature, wild type
How to Cite
Downloads
References
Abiodun O. Biofuel opportunity and development of renewable energies markets in Africa: A paper presented during the biofuel market Africa 2007 conference. Cape Town, South Africa. 2007;10-5.
International Energy Agency. Summary World Energy Outlook 2022; 2022 [cited on Jul 20, 2023].
Available:http://www.iea.org
Abila N. Biofuels adaption in Nigeria: A preliminary review of feedstock and fuel production potential. Finland: Department of Industrial Management University of Vaasa. 2010;1-11.
Ho DP, Ngo HH, Guo W. A mini review on renewable sources for biofuel. Bioresour Technol. 2014;169:742-9.
Jambo SA, Abdulla R, Mohd Azhar SH, Marbawi H, Gansau JA, Ravindra P. A review on third generation bioethanol feedstock. Renew Sustain Energy Rev. 2016;65:756-69.
Pan W, Xi L. Advances in 2nd generation of bioethanol production. A volune in Woodhead Publishing Series in Energy. 2021;1-7.
Ado SD, Kachalla GU, Tijjani MB, Aliyu MS. Ethanol production from corn cobs by co-cultures of Saccharomyces cerevisiae and Aspergillus niger. Bayero J Pure App Sci. 2009;2(2):99-101.
Singh A, Pant D, Korres NE, Nizami AS, Prasad S, Murphy JD. Key issues in life cycle assessment of ethanol production from lignocellulosic biomass: Challenges and perspectives. Bioresour Technol. 2010;101(13):5003-12.
Melekwe EI, Lateef SA, Rowland G, Ekpeyong E. Bioethanol production potentials of corn cob, waste, office paper and leaf of Thaumatococcus daniellii. Bayero J Appl Sci Technol. 2016;17(4):1-10.
Budzianowski MW. High-value, low-volume bioproducts coupled to bioenergies with potential to enhance business development of sustainable bio- refineries. Renew Sustain Energy Rev. 2017;70:799-804.
Zhao X, Zhang L, Liu D. Biomass recalcitrance. Part I: The chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose. Biofuels Bioprod Bioref. 2012;6(4):465-82.
Adegbehingbe KT, Faparusi F, Adeleke BS. Bioethanol production from Cassava peels inoculated with Saccharomyces cerevisiae and Zymomonas mobilis. Adv Microbiol. 2021;21(9):58-67.
Achinas S, Euverink GJW. Consolidated briefing of biochemical ethanol production from lignocellulosic biomass. Electron J Biotechnol. 2016;23:44-53.
Okafor N. Modern industrial microbiology and biotechnology. Enfield: Science Publishers. 2007;126-48.
Shinde SA, Chavhan SA, Sapkal SB, Shrikhade VN. Recombinant DNA technology and its application [review]. Int J Medipharm Res. 2018;04(2):77-80.
Ballesteros I, Ballesteros M, Manzanares P. Dilute sulfuric acid pretreatment of Carson for ethanol production. J Biochem Eng. 2008;42:84-91.
Robak K, Balcerek M. Review of second generation bioethanol production from residual biomass. Food Technol Biotechnol. 2018;56(2):174-87.
Oyeleke SB, Jibrin NM. Production of bioethanol from guinea corn husk and millet husk. Afr J Microbiol. 2009;3(4):147-52.
Oyeleke SB, Manga SB. Essential of laboratory practical in microbiology. 1st ed,Tobest. Vol. 2009. Minna, Nigeria. 2008;36-69.
Adetuwo OJ. Evaluation of the microbiological quality and safety of pupuru and garri on sale at Okitipupa main market in Okitipupa Local Government Area, Ondo, Nigeria. Am J Food Sci Nutr. 2020;2(1):32-44.
Sawalha H. Laboratory manual of mycology and plant pathogenic fungi. Al-Jamea’yah Bookshop Press. Nablus, Palestine. 2014;1-66.
Yeast Data Base [cited Jan 10, 2023]. Available:https://theyeasts.org
Singh J, Sharma R. Kinetic and modeling of ethanol production by wild and mutant S. cerevisiae MTCC 170. Eur J Exp Biol. 2015;5(4):1-6.
Liu JJ, Ding WT, Zhang GC, Wang JY. Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination. Appl Microbiol Biotechnol. 2011;91(4):1239-46.
Ababio OY. New senior secondary school chemistry. Africana publishers PLC. Nigeria: Onisha. 2018;350-400.
Ogbeibu AE. Biostatistics: A practical approach to research and data handling. 3rd ed. Benin-City, Nigeria: Mindex Publishing. 2015;17-128.
Alvira P, Negro MJ, Ballesteros I, González A, Ballesteros M. Steam explosion for wheat straw pretreatment for sugars production. Bioethanol. 2016;2(1):66-75.
Sarris D, Papanikolaou S. Biotechnological production of ethanol: Biochemistry, processes and technologies. Eng Life Sci. 2016;16(4):307-29.
Gunasekaran P, Chandra KR. Ethanol fermentation technology: M. mobilis. Madurai, India: Madura Kamary University. 2007;1-22.
Crook N, Alper HS. Classical strain improvement. In: Patnaik R, editor. Engineering complex phenotypes in industrial strains. Hoboken, NJ: John Wiley & Sons, Inc. 2012;1-33.
Hashimoto S, Ogura M, Aritomi K, Hoshida H, Nishizawa Y, Akada R. Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis. Appl Environ Microbiol. 2005;71(1):312-9.
Hockberger PE. A history of ultraviolet photobiology for humans, animals and microorganisms. Photochem Photobiol. 2002;76(6):561-79.
Kumari R, Pramanik K. Improvement of multiple stress tolerance in yeast strain by sequential mutagenesis for enhanced bioethanol production. J Biosci Bioeng. 2012;114(6):622-9.
Stanley D, Bandara A, Fraser S, Chambers PJ, Stanley GA. The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae. J Appl Microbiol. 2010;109(1):13-24.
Khattak WA, Ul-Islam M, Ullah MW, Yu B, Khan S, Park JK. Yeast cell-free enzyme system for bio-ethanol production at elevated temperatures. Process Biochem. 2014;49(3):357-64.
Buijs NA, Siewers V, Nielsen J. Advanced biofuel production by the yeast Saccharomyces cerevisiae. Curr Opin Chem Biol. 2013;17(3):480-8.
Almeida P, Barbosa R, Zalar P, Imanishi Y, Shimizu K, Turchetti B et al. A population genomics insight into the Mediterranean origins of wine yeast domestication. Mol Ecol. 2015;24(21):5412-27.
Ashenafi M. A review on the microbiology of indigenous fermented foods and beverages of Ethiopia. Ethiop J Biol Sci. 2008;5:189-245.
Hardison R. Mutations and mutagens. PA: The Pennsylvania State University Press. 2021;1-60.
Kim S, Dale BE. Global potential bioethanol production from wasted crops and crops residues. Biomass Bioenergy. 2004;26(4):361-75.
Rabbani G. Involvement of mutagens in the production of bioethanol by Saccharomyces cerevisiae: A review. J Environ Anal Chem. 2018;05(3):1-4.
Singh S, Chakravarty I, Kundu S. Mathematical modelling of bioethanol production from algal starch hydrolysate by Saccharomyces cerevisiae. Cell Mol Biol (Noisy-le-grand). 2017;63(6):83-7.
Udom N, Chansongkrow P, Charoensawan V, Auesukaree C. Coordination of the cell wall integrity and high-osmolarity glycerol pathways in response to ethanol stress in Saccharomyces cerevisiae. Appl Environ Microbiol. 2019;85(15): 21-56.
Zhang W, Geng A. Improved ethanol production by a xylose-fermenting recombinant yeast strain constructed through a modified genome shuffling method. Biotechnol Biofuels. 2012;5(1):46.