Efficacy of Azadirachta indica in the Treatment of Gastrointestinal Helminthiasis

Adams E. G.

Department of Science Laboratory Technology (Environmental Biology), Heritage Polytechnic, Eket, Nigeria.

Ekott E. J. *

Department of Science Laboratory Technology, Heritage Polytechnic, Eket, Nigeria.

Usip L. P.

Department of Biological Science, Akwa Ibom State Polytechnic, Ikot Osura, Nigeria.

Opara K. N.

Department of Animal and Environmental Biology, University of Uyo, Akwa Ibom State, Nigeria.

*Author to whom correspondence should be addressed.


The study was carried out to assess the anthelminthic efficacy of Azadirachta indica commonly known as Neem (Dogoyaro). The phytochemical components of the leaf extract was accessed using Gas Chromatography Mass Spectrometry and the efficacy was carried out in vitro and in vivo using Toxocara canis (as worm model) of dogs. Twenty peaks of compounds were revealed in the GS-MS chromatogram, with Hexadecanoic acid which is saddled with anthelminthic effect as one of its major constituents. In the in vitro study, the lethal concentration was 92.48ml/mg at 6hrs. Percentage egg reduction of 76.2% and 83.9% from the Faecal Egg Count (FEC) at LC25 and LC50 respectively was recorded for the in vivo study. The overall blood count of all the puppies used for the experiment were 4.46 X 106µL for RBC, 31.32% for PCV and 11.23 X 103for WBC. Puppies treated with Azadirachta indica leaf extract showed minimal alteration of the three heamatological parameters (WBC, PCV and RBC) analyzed compared to the uninfected control group and group treated with Mbendazole. This indicates that Azadirachta indica leaf extract is effective in the treatment of gastrointestinal helminthiasis and also further investigations are recommended as it may be useful in the treatment of other diseases.

Keywords: Efficacy, gastrointestinal helminthiasis, phytochemical, anthelminthic

How to Cite

E. G., A., E. J., E., L. P., U., & K. N., O. (2023). Efficacy of Azadirachta indica in the Treatment of Gastrointestinal Helminthiasis. Biotechnology Journal International, 27(5), 47–55. https://doi.org/10.9734/bji/2023/v27i5695


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Papaiakovou M. Timothy D, Littlewood J, Doyle SR, Gasser RB, Cantacessi C. Worms and Bugs of the Gut: The Search for Diagnostic Signatures Using Barcoding, and Metagenomics–Metabolomics. Parasites & Vectors. 2022;15(118): 2-13.

Kajero OT, Janoušková E, Bakare EA, Belizario V, Allen DB, Alonte J, Manalod SM, Paller V G, Betson M, Prada JM. Co-Infection of Intestinal Helminths in Humans and Animal in the Philippines. Trans R Soc Trop Med Hyg. 2022;116:727–735.

Schafer TW, Skopic A. Parasites of the Small Intestine. Curr Infect Dis Rep. 2007; 9:60–68.


Carlson CJ, Dallas TA, Alexander LW, Phelan AL, Phillips AJ. What would it take to describe the global diversity of parasites? Proc. R. Soc. 2020;287.

Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, Diemert D, Hotez PJ. Soil-Transmitted Helminth Infections: Ascariasis, Trichuriasis and Hookworm. Lancet. 2006;367:1521-1532.

Centre for Disease Control (CDC) Soil Transmitted Helminths: Causes, prevention and control.

Available:http://www.cdc.gov/parasites/sth/Accessed on January. 10:2013.

Hotez PJ, Asojo OA, Adesina AM. Nigeria: Ground Zero for the High Prevalence Neglected Tropical Diseases. Plos Neglected Tropical Disease. 2012;6(7): 1600-1605.

Ketzis JK, Tayler A, Brown DD, Brwon DA, Warnick LD, Erb HN. Chenopodium ambrosioides and its Essential oil as Treatments for Haemonchus contortus and Mixed Adult-nematode Infections in Goats. Small Rumin. Res. 2002;44:193–200.

Githiori JB, Athansiadou S, Thamsborg SM. Use of Plants Novel Approaches for Control of Gastro-Intestinal Helminths in Livestock with Emphasis on Small Ruminants. Vet. Parasitol. 2006;139:308–320.

Eguale T, Tilahun G, Debella A, Fleke A, Makonnen E. Haemonchus contortus: In vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix. Experim. Parasitol. 2007; 116:340–345.

Wilcox RS, Bowman DD, Barr SC, Euclid JM. Intestinal Obstruction Caused by Taenia Taeniformis Infection in Cat. J Am Anim Hosp Assoc. 2009;45(2):93–96.

Otubanjo O. Parasites of Man and Animals. First Edition. Concept Publication Limited. Lagos, Nigeria. 2013.

Campbell S, Soman-Faulkner K. Antiparasitic Drugs. In: Statpearls [Internet]. Treasure Island (FL): Statpearls Publishing; 2023. Available:https://www.ncbi.nlm.nih.gov/books/nbk544251/

Rosenthal PJ. Clinical pharmacology of the antihelminthic drugs. Katzung BG, (Ed.), Basic and Clinical Pharmacology. 14e. Mcgraw Hill; 2017. Available:https://Accessmedicine.Mhmedical.Com/Content.Aspx?Bookid=2249&Sectionid=175224314

Fernandes FD, Guerra RR, Ries SA, Cargnelutti FJ, Sangioni AL, Vogel FSF. Gastrointestinal Helminths in Dogs: Occurrence, Risk Factors, and Multiple Antiparasitic Drug Resistance. Parasitology Research; 2022. Available:https://Doi.Org/10.1007/S00436-022-07599-0

WHO Reasearch Priorities for Helminth Infections. Technical Report Series, No 972. Geneva; 2012.

Available:https://www.who.int/publications-detail-redirect/WHO-TRS-972/retrieved on 09/06/2022

Jackson F, Coop RI. The Development of Anthelminthic Resistance in Sheep Nematode. Parasitology. 2000;120:95-107.

Sangaster NC. Anthelminthic Resistance: Past, Present and Future. International Journal of Parasitology. 2008;29:115-124.

Marsh AE, Lakritz J. Reflecting on the past and fast forwarding to present day anthelmintic resistant ancylostoma caninum: A Critical Issue We Neglected To Forecast. International Journal of Parasitology; Drugs and Drug Resistance. 2023;22:36-43.

Tandon V, Yadav AK, Roy B, Das B. Emerging trends in zoology: Phytochemicals as cure for worm infections in traditional medicine system. Narendra Publication House, New Delhi. 2011;351-378.

Al-Shaibani IRM, Phulan MS, Shiekh M. Anthelmintic activity of Fumaria parviflora (Fumariaceae) against gastrointestinal nematodes of sheep. Int. J. Agric. Biol. 2009;11(4):431–436.

Fajmi AK, Taiwo AA. Herbal Remedies in Animal Parasitic Diseases in Nigeria: A Review. Afr J Biotec. 2005;4:303-7.

Eguale T, Giday M. In vitro anthelmintic activity of three medicinal plants against Haemonchus contortus. International Journal of Green Pharmacy. 2009;29-34.

Athanasiadou S, Githiori J, I Kyriazakis I. Medicinal plants for helminth parasite control: Facts and Fiction. Animal 2007; 1(9):1392–1400.

Porter AH. Neem: India’s Tree of Life. BBC News;2006.


Access on 09/06/2022

Sujon MA, Mostofa M, Jahan MS, Das AR, Rob S. Studies on medicinal plants against gastroinstestinal nematodes of goats. Bangl. J. Vet. Med. 2008;6(2):179–183.

Oshiobugie MJ, Olaniyi AM, Raphael AO. AAS and GC-MS Analysis of Phytocomponents in the Leaf, Stem and Root of Azadirachta indica A. Juss (Dongoyaro). British Journal of Pharmaceutical Research. 2017;15(4):1-12.

AAT Bioquest Inc. Quest Graph LC50 Calculator. Available:http://www.aatbiio.com/tools/LC50-Calculator

Access on 2022;23:06.

Salien AM, Kassuku AA, Magwisha H, Edward C, Marisa J, Ngovi C. Determination of anthelminthic resistance in goats and sheep using faecal egg count reduction test at Luguruni Farm, Dares Salaam, Tanzania. Tazania Veterinary Association Proceedings. 2019;34:87- 90.

Nweze NE, Arine O, Ngongeh LA. Anthelmintic Potential of Three Plants Used in Nigeria Ethnoveterinary Medicine. Pharmaceutical Biology. 2013;5(3):311-315.

Nambiar BK, Premaalatha B, Chandrawathani P, Zary ST. Efficacy of Neem and Sbah Snake Grass Leaves Water Extract and Decoction against Grastrointestinal Parasites in Local Goats. Malaysian Journal of Veterinary Research. 2016;7(2):91-101.

Dele OS, Arowosegbe S, Oyegun BA. phytochemical properties of Azadirachta indica (A. Juss) harvested at different times of the day. International Journal of Herbal Medicine. 2020;8(2):7-11.

Aswathi PV, Dhivya R. Quality phytochemical screening and mosquito replelency of Chromolaena odonrata leaf extract against adult Culex sp. Indo American Journal of Pharmaceutical Science. 2017;4(3):698-705.

Ujah I, Nsude C, Ani O, Alozieuwa U, Okwor A, Okpako I. Phytochemicals of neem plant (Azadirachta Indica) explains its use in traditional medicine and pest control. gsc biological and pharmaceutical sciences. 14. 10.30574/Gscbps. 2021; 14(2):0394.

Arona C, Tamakar V. Gmelina arborea: Chemical Constituents, Pharmacological Activities and Applications. Journal of Phytomedicine. 2017;9:528-542.

Abirami P, Rajendran A. GC-MS Analysis of Methanol Extracts of Vernonia cinerea. European Journal of Experimental Biology. 2012;2(1):9-12.

Alzohairy MA. Therapeutics Role of Azadirachta Indica (Neem) and their Active Constituents in Diseases Prevention and Treatment. Evidence-Based Complemen-tary and Alternative Medicine. 2016;2016 Article ID 7382506, 11 Pages, 2016. (2013). Available:https://Doi.Org/10.1155/2016/7382506