Isolation, Characterization, and GC-MS Analysis of Fatty Acid of Mugil parsia Fish Oil

Shahanaz Khatun *

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh.

*Author to whom correspondence should be addressed.


This paper reports the isolation, characterization, and GC-MS analysis of Mugil parsia fish oil collected from the Rupsha River in Khulna. The oil was extracted from the dried fish by using the Soxhlet apparatus with n-hexane, with an oil yield of 17± 4.0 %. The moisture, ash, and protein content of the raw fish were found to be 75.45 %, 1.258 %, and 20.00 % respectively. The physicochemical compositions of the extracted oil like color, specific gravity, peroxide value, saponification value, free fatty acid (FFA), acid value, and iodine value were determined and were found to be yellowish brown in color, 0.913±0.31, 9.9±0.1 meq/kg, 295.4±3.0 mg KOH/g, 11.9±2.0%, 5.7±0.11mg KOH/g, and 150.3±0.12 mg/100g respectively.  Fatty acid composition was identified by GC-MS analysis of the Mugil parsia fish oil, showed the Phthalic acid, mono-(2-ethylhexyl) ester, palmitic acid, oleic acid, stearic acid, icosanoic anhydride, glycidyl stearate were identified as the major constituents.

Keywords: Mugil parsia, soxhlet apparatus, peroxide value, iodine value, GC-MS analysis

How to Cite

Khatun , S. (2023). Isolation, Characterization, and GC-MS Analysis of Fatty Acid of Mugil parsia Fish Oil. Biotechnology Journal International, 27(5), 56–63.


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Helfman. Microbiological and physiochemical level of fresh catfish (Arius hendolitic) fromdifferent Markets in Akwa-Ibom State, Nigeria. New York Science Journal. 19975(4):46-52.

Aryee A, Simpson B. Comparative studies on yield and quality of solvent-extracted oil from Salmon skin. Journal of Food Engineering. 2009;92(3):353-358.

Exler J. Composition of foods: Finfish and shellfish products. United State department of agriculture, Human Nutrition Information Service, Agriculture Handbook 8-15 (Updated 1992) Washington. DC. 1987; 192.

Homayooni B, Sahari MA.,and Barzegar M. Concentrations of omega-3 fatty acids from rainbow sardine fish oil by various methods. International Food Research Journal.2014; 21(2) : 743-748.

Ndidiamaka NC, Ifeanyi OE. Proximate and physicochemical analysis of oil obtained from two fish species (fresh and frozen). International Journal of Advanced Research in Biological Sciences. 2018; 5(4):167-77.

Sidhu KS. Health benefits and potential risks related to consumption of fish oil or fish. Journal of Regulatory Toxicology and Pharmacology. 2003; 38:336-344.

Arthritis . fish oil for arthritis and joint pain: Can It Help? The omega-3 in fish oil may block some sources of inflammation. By Yvelette Stines ,Updated on May 09, 2023.Medically reviewed by Mohamad Hassan, PT.


Mahmud I, Anwar H, Shahdat H, Abdul H, Liaquat A, Hashimoto M. Effects of Hilsa ilisa fish oil on the atherogenic lipid profile and glycaemic status of streptozotocin-treated type 1 diabetic rats. Clinical and Experimental Pharmacology and Physiology. 2004;31:76–81.

Castro R, Queirós J, Fonseca I, Pimentel JP, Henriques AC, Sarmento AM, Guimarães S, Pereira MC. Therapy of post-renal transplantation hyperlipidaemia: Comparative study with simvastatin and fish oil. Nephrol Dial Transplant. 1997 Oct; 12(10):2140-3. DOI:10.1093/ndt/12.10.2140. PMID: 9351079.

Anil K, Jayadeep A, Sudhakaran PR. Effect of n-3 fatty acids on VLDL production by hepatocytes is mediated through prostaglandins. Biochem Mol Biol Int. 1997 Dec;43(5):1071-80.

DOI: 10.1080/15216549700204891. PMID: 9415816.

Van Vlijmen BJ, Mensink RP, Van't-Hof HB, O_ermans RF, Hofker MH, Havekes LM, J. Lipid. Res. 1998;39:1181.

Ariza-Ariza R, Mestanza-Peralta M, Cardiel MH. Omega-3 fatty acids in rheumatoid arthritis: An overview. Semin Arthritis Rheum. 1998 Jun;27(6):366-70.

DOI:10.1016/s0049-0172(98)80016-4. PMID: 9662755.

James MJ, Cleland LG. Dietary n-3 fatty acids and therapy for rheumatoid arthritis. Semin Arthritis Rheum. 1997 Oct;27(2):85-97. DOI:10.1016/s0049-0172(97)80009-1. PMID: 9355207.

Gonnerman WA, Mortensen RF, Tebo JM, Conte JM, Leslie CA, Edgar S, Immunol J. 1988;140:796.

Manitius J, Sulikowska B, Fox J, Jakubowski Z, Ludwiczak E, Lysiak-Szydłowska W, Rutkowski B. The effect of dietary enrichment with fish-oil on urinary excretion of N-acetyl-beta-D-glucosaminidase and renal function in proteinuric patients with primary glomerulopathies. Int Urol Nephrol. 1997;29(4):489-95.

DOI: 10.1007/BF02551118. PMID: 9406009.

Dillon JJ. Fish oil therapy for IgA nephropathy: Efficacy and interstudy variability. J Am Soc Nephrol. 1997 Nov; 8(11):1739-44.

DOI: 10.1681/ASN.V8111739. PMID: 9355077.

Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497-509. PMID: 13428781.

Alexandre L. Craig LJ. .Reevalution of the DHA requirement for premature infant. Prostanglandins,Leukotrienes and Essential Fatty Acids. 2009;81:143-150.

Dexuan , Minmin Z, Christain P. DHAPromotes the Neuronal Differentiation of rat Neural Stem Cells Transfected with GPR40 gene. Brain Research; 2010.

AOCS In: AOCS (ed) Official methods & recommended practices of the American Oil Chemists Society, 4th edn. Champaign, IL,Official Method Ai 275; 1993.

Lowry OH, Rosebrough NJ, Farr AL, Randal RJ. Protein measurement with the Folin-Ciocalteu’s reagent. J. Biol.Chem. 1951;193:265-275.

AOCS. Official methods and recommended practices of the American Oil Chemists Society (4th ed.), Champaign, American Oil Chemists’ Society, USA; 1992.

AOAC. Official methods of analysis of the Association of Official Analytical Chemists (14th ed.), Arlington; 2002.

Steel RGD, Torrie JH, Dickey DA. Principals and procedure of statistics. McGraw Hill Book Co. Inc., New York; 1997.

Pike OA. Fat characterization in food analysis, (3/e), Aspen Pub.Inc. 1998;219-233.

Young S, Sarda X, Rosenberg M. Microencapsulating properties of whey proteins. 1. Microencapsulation of Anhydrous Milk Fat. Journal of Dairy Science. 1993;76(10);2868-2877. Available:

Bimbo AP, Crowther JB. Fish oil: Processing beyond crude oil. INFOFISH International. 1991;6(91):20-25.

Chantachum S, Benjakul S, Sriwirat N. Separation and quality of fish oil from precooked and non-precooked tuna heads. Food Chemistry. 2000;69:289-294.

Ashraduzzaman M, Alam MA, Khatun S, Bari L. and Absar N. Extraction and characterization of Vigna unguiculata Linn. walp. seed oil, Journal of Science and technology. 2013:3(2):431-438.

Dauksas E, Falch E, Slizyte R, Rustad T. Composition of fatty acids and lipid classes in bulk products generated during enzymic hydrolysis of cod (Gadus morhua) by-products, Process Biochemistry. 2005;40: 2659-2670.

Merck & Co "You Can Control Your Cholesterol: A Guide to Low-Cholesterol Living". Inc. Retrieved; 2009-03-14.

Teres S, Barcelo-Coblijn G, Benet, M, Alvarez, R, Bressani R, Halver JE, Escriba PV. Oleic acid content is responsible for the reduction in blood pressure induced by olive oil. Proceedings of the National Academy of Sciences. 2008;105(37): 13811–6.

Bibcode:2008PNAS.10513811T. DOI:10.1073/pnas.0807500105. JSTOR 25464133. PMC 2544536 . PMID 18772370.

Mensink RP. Effects of stearic acid in plasma lipid and lipoproteins in human. Lipids. 2005;40 (12):1201-5.

Das UN. Arachidonic acid in health and disease with focus on hypertension and diabetes mellitus: A review. J Adv Res. 2018 Jan 4;11:43-55. DOI:10.1016/j.jare.2018.01.002. PMID: 30034875; PMCID: PMC6052660.