Biomolecular Characterization of Lactic Acid Bacteria (Lactobacilli and Cocci) Isolated from Nonnonkoumou, a Artisanal Milk Curd in Daloa, Côte D’ivoire

Dogoua Koko Guillaine Jostasie *

Department of Biochemistry and Microbiology, Agrovalorisation Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire and Department of Food Science and Technology, Laboratory of Food Biotechnology and Microbiology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire.

Assohoun-Djeni Nanouman Marina Christelle

Department of Food Science and Technology, Laboratory of Food Biotechnology and Microbiology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire.

Kouassi Kouassi Clément

Department of Biochemistry and Microbiology, Agrovalorisation Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire.

Djeni N’dédé Théodore

Department of Food Science and Technology, Laboratory of Food Biotechnology and Microbiology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire.

*Author to whom correspondence should be addressed.


Abstract

Nonnonkoumou is an artisanal sour milk consumed in Côte d'Ivoire, particularly in the town of Daloa. Its production is carried out under uncontrolled conditions and uses a variety of lactic acid bacteria. The main objective of this study was to characterize the cocci and lactobacilli present in the nonnonkoumou. The cocci had the highest loads going from 8.31 ± 0.14 log CFU / mL to 6.76 ± 0.01 log CFU / mL compared to the lactobacilli which had loads between 6.12 ± 0.30 log CFU / mL and 4.35 ± 0.42 log CFU / ml. This preponderance of cocci in all the nonnonkoumou samples taken in comparison to bacilli have been shown through the cocci / bacilli ratio which varied from 1.9 to 1.22. PCR followed by Amplified Ribosomal DNA Restriction Analysis (ARDRA) after enzymatic digestion with Hae III and Hinf I have revealed 4 Hinf I profiles and 3 Hae III for lactobacilli and 2 Hae III profiles and 4 Hinf I profiles for cocci. This technique has revealed 4 species of lactobacilli isolated from nonnonkoumou which are Lactobacillus reuteri, Lactobacillus paracasei, Lactobacillus plantarum and Fructobacillus durionis and 6 groups of cocci corresponding to 6 species. The species identified during this study made it possible to understand their technological role and their contribution to the health quality of nonnonkoumou.

Keywords: Milk, nonnonkoumou, fermentation, lactic acid bacteria, PCR-ARDRA


How to Cite

Jostasie, D. K. G., Christelle , A.-D. N. M., Clément, K. K., & Théodore, D. N. (2024). Biomolecular Characterization of Lactic Acid Bacteria (Lactobacilli and Cocci) Isolated from Nonnonkoumou, a Artisanal Milk Curd in Daloa, Côte D’ivoire. Biotechnology Journal International, 28(1), 9–20. https://doi.org/10.9734/bji/2024/v28i1710

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References

Mirah-dpp. Annuaire des statistiques des ressources animales et halieutiques. Direction de la Planification et des programmations, Ministère de Ressources Animales et Halieutiques, Abidjan, Côte d’Ivoire. 2012;26.

Djermane I. Enumeration, isolation and identification of antibacterial lactic acid bacteria isolated from milk and traditional Bouhezza goat cheese. Master in Biology Option: Applied Microbiology. Universite Larbi Ben M'hidi Oum El-Bouaghi. 2020; 61.

Jespersen L, Halm M, Kpodo K, Jacobson M. Significance of yeasts and moulds occurring in maise dough fermentation for kenkey production Int. J. Food Microbiol. 1994;24:239-248.

Latreche B. Characterisation of lactic bacteria isolated from raw butter, evaluation of their technological properties and their use in the manufacture of sour cream. Master in Food Science Option: Food Technology. Institute of Nutrition, Food and Agri-Food Technologies Department of Food Technology. 2016; 150.

Sansanwal R, Ahlawat U, Dhanker R, Yoghurt: A predigested food for lactose-intolerant people. Int. J. Curr. Microbiol. Appl. Sci. 2017;6(12):1408-1418.

Mishra C, Lambert J. Production of anti-microbial substances by probiotics. Asia Pac J Clin Nutr. 1996;5(1):20-4.

Piard J, Desmazeaud M. Inhibiting factors produced by lactic acid bacteria. 2. Bacteriocins and other antibacterial substances. Lait. 1992 ;72 :113-142.

Assohoun-Djeni NMC, Kouassi KC, Kouassi KA, Yao KC, Djeni NT, Konate I. Physicochemical Characteristics and Microbial Contamination of Raw Milk and Artisanal Milk Curd (Nonnonkoumou) in Daloa, Côte D’Ivoire. Int. J. Curr. Microbiol. Appl. Sci. 2020;9(03):2667-2683.

Feutry F. Dynamics of lactic bacteria in Ossau-Iraty PDO cheese made from raw milk: Use of wild strains of Lactococcus lactis in farmhouse production. Doctoral Thesis. Public University of Navarre, 2016; 149.

Morandi S, Brasca M, Lodi R. Technological, phenotypic and genotypic characterization of wild lactic acid bacteria involved in the production of Bitto PDO Italian cheese,” Dairy Sci. Technol. 2011; 91(3):341-359.

Coulin P, Farah Assanvo J, Spillman H, Puhan Z. Characterization of the microflora of attiéké, a fermented cassava product during traditional small-scale production. Int. J. Food Microbiol. 2006;106:131-136.

Prescott M, Nowakowski S, Gavin P, Naglev P, Whisstock J, Devenish R. Subunit gamma-green fluorescent protein fusions are functionally incorporated into mitochondrial F1F0-ATP synthase, arguing against a rigid cap structure at the top of F1. Journal of Biology Chemical. 2003; 278:251-6.

Hassaïne O, Zadi-karam H, Karam N. Evaluation of biogenic amines formation by proteolytic enterococci strains isolated from raw dromedary milks from southern Algeria. Journal of Food Safety. 2008;29: 381-393.

Weisburg WG, Barms SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 1991; 173:697-703.

Attchelouwa CK, N’guessan FK, Aké FMD, Marcellin KD. Molecular identification of yeast, lactic and acetic acid bacteria species during spoilage of tchapalo, a traditional sorghum beer from Côte d’Ivoire. W. J. Microbiol. Biotech. 2018;34: 173-183.

Kim M, Chun J. Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis. International Journal of Food Microbiology. 2005;103(1):91-96.

Ouazzani TN, Arfaoui A. Fadli M. Evaluation de la qualité microbiologique du lait cru dans la région du Gharb, Maroc. Inter. J. Inn. and Scientific Res. 2014;9: 487-493.

Zamfir M, Vancanneyt M, Makras L, Vaningelgem F, Lefebvre K, Pot B, Swings J. De Vuyst L. Biodiversity of lactic acid bacteria in Romanian dairy products. Syst. Appl. Microbiol. 2006;29:487-495.

Ren Y, Liu W, Zhang H. Identification of Coccoidal Bacteria in Traditional Fermented Milk Products from Mongolia, and the Fermentation Properties of the Predominant Species, Streptococcus thermophilus. Korean J Food Sci Anim Resour. 2015;35:683-391.

Ndiaye M. Contribution à l’étude de conformité de l’étiquetage de la qualité microbiologique des yaourts commercialisés à Dakar. Thèse de Doctorat, Université Cheikh AntaDiop de Dakar, Dakar. 2002;11.

Christelle S, Sylvie B, Chadi H, Joel S. An overview of recent studies on the main traditional fermented milks and white cheeses of the Mediterranean region. Food reviews International. 2016;32(3).

Erkuş O. Isolation, phenotypic and genotypic characterization of yoghurt start bacteria. Master of science In Food Engineering, Tukey. 2007;117.

Bozoudi D, Agathokleous M, Anastasiou I, Papademas P, Tsaltas D. Microbiological characteristics of trachanas, a Traditional Fermented Dairy Product from Cyprus. J. Food Qual. 2017;1-6.

Shah N. The product and its manufacture. In encylopedia of food science and nutrition. Edited by Cabarello B, Trugo L, Finglas P. London: Academic Press. 2003; 6252-6262

Amoroso S, Robert S, Giovani L, Alessandro P, Nadia L, Giuseppe A, Paolo F, Domonico G. Identification des allergènes par analyse in vivo et in vitro. Archives internationales d'allergie et d'immunologie appliquée. 1988;85(1):69-75.

Blaiotta G, Fusco V, Ercolini D, Aponte M, Pepe O, Villani F. Lactobacillus strain diversity based on partial hsp60 gene sequences and design of PCR- restriction fragment length polymorphism assays for species identification and differentiation, Appl. Environ. Microbiol. 2008;74:208-215.

Stackebrandt E, Goebel BM. Taxonomic note: A place for DNA- DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 1994;44:846-849

Yu Y, Song C, Zhang Q, Dimaggio PA, Garcia BA, York A, Carey MF, Grunstein M. Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA. Mollen Cell. 2012;46(1):7-17

Afaf IS. Molecular identification of probiotics lactobacillus Strain isolates by: Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sci. J. Microbiol. 2012; 6(12):3034-3041.

Kunene NF, Ifigenia G, Geornaras I, Von Holy A, Hastings JW. Characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting. Appl. Environ. Microbiol. 2000;66:1084-1092.

Kunal D, Ruplal C, Katherine A, Thompson W. Effects of new technology on the current yogurt manufacturing process - to increase the overall market value of yogurt. LWT. 2019 ;69-80.

Leory F, Degeest B, De vuyst L. A novel area of predictive modeling: describing the functionally of beneficial micro-organisms in foods. Int. J. Food Microbiol. 2002;73: 251-259.

Assam T, Idiri F. Trial for the manufacture of flavoured stirred yoghurts based on cow's milk and soya milk. Comparative study of different parameters. Master In Human Food And Product Quality, Université Mouloud Mammeri de Tizi Ouzou. 2015;96.

Seki M, Igarashi M, Fukuda Y, Simamura S, Kawashima T, Ogasa K. The effect of Biftdobacterium cultured milk on the 'regularity' among the aged group. J Jpn Soc Food Nutrit. 1978;31:379-387.

Hughes DB, Hoover DG. Bifidobacteria: Their potential for use in American dairy products. Food Technol. 1991;45(4):74-83.

O’Sullivan M.G., Thornton G., O’Sullivan G.C. & Collins J.K. Probiotic bacteria: myth or reality? Trends Food Sci. Technol. 1992;3:309-314.

Rekha S, Umang A et Rinku D. International Journal of Current Microbiology and Applied Sciences. 2017; 6(12):1408-1418 . ISSN: 2319-7706

Khelili D. Effect of probiotics and prebiotics on viral infections. Master en Sciences Alimentaires Option: Agro-alimentaire et contrôle de qualité, Université Mouloud Mammeri de Tizi-Ouzou, 2020;83.

Bhat Z, Bhat H. Milk and dairy Products as Functional Foods: A review. Int. J. Dairy Sci. 2011;6(1):1-12.