Optimization of Plant Growth Regulators in Indirect Plant Regeneration in Rice Variety Nerica 3 Using Mature Seeds as Explant
Danielle Christelle Tinak Ekom *
Mbalmayo Agricultural Research Centre, Institute of Agricultural Research for Development (IRAD), P.O. Box 2067, Yaounde, Cameroon.
Haïcha Diko Abba
Garoua Multipurpose Research Station, Institute of Agricultural Research for Development (IRAD), P.O. Box 415, Garoua, Cameroon.
Jean Cyrille Mouen Piau
Jay P. Johnson Biotechnology Laboratory, Ekona Research Centre, Institute of Agricultural Research for Development (IRAD), PMB 25, Buea, Cameroon.
Hermine Claudine Mahot
Mbalmayo Agricultural Research Centre, Institute of Agricultural Research for Development (IRAD), P.O. Box 2067, Yaounde, Cameroon.
Nathalie Gaelle Tinak Satok
Ministry of Livestocks, Fisheries and Animal, P.O. Box- 20355, Yaounde, Cameroon.
Rodrigue Simonet Poueme Namegni
Epidemiology and Public Health Service, Centre Pasteur du Cameroun (CPC), P.O. Box 1274, Yaounde, Cameroon.
Abel Wade
National Veterinary Laboratory (LANAVET), P.O. Box 503, Garoua, Cameroon.
*Author to whom correspondence should be addressed.
Abstract
The study is the first attempt to highlight about callus induction and plantlet regeneration in rice variety ‘Nerica 3’ using mature seed as explant and using five regeneration media with plant growth regulators (BA, IAA, and 2,4-D) at different concentrations in other to establish the efficient indirect plant regeneration protocol. Among the five regeneration media tested, R3 medium (2 mg/L BA), showed greater percentage of plantlet regeneration (45.31%) followed by R2 medium (0.5 mg/L BA + 0,1 mg/L IAA; 29.68%), whereas, R4 medium showed the lowest (2 mg/L 2,4-D; 0%). These results will be used for genetic improvement programs of the variety ‘Nerica 3’.
Keywords: Rice variety ‘Nerica 3’, mature seed, plantlet regeneration, Plant growth regulators