Biotechnology Journal International https://journalbji.com/index.php/BJI <p style="text-align: justify;"><strong>Biotechnology Journal International (ISSN:&nbsp;2456-7051)</strong> publishes original research papers, review articles and short communications on all areas of Biotechnology including cell biology, genetics, microbiology, immunology, molecular biology, biochemistry, embryology,&nbsp; immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, pharmacogenomics, gene therapy, plant, animal, microbial and environmental biotechnology.&nbsp;The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Biotechnology Journal International 2456-7051 Effects of Cadmium Stress on Photosynthetic Physiological Characteristics of Two Rorippa Species https://journalbji.com/index.php/BJI/article/view/30120 <p>The photosynthetic physiological characteristics of <em>Rorippa amphilia</em> and <em>Rorippa sylvestris</em> were studied by pot experiment under Cd stress, and 7 Cd treatments were 0, 1, 5, 10, 50, 100, 200(mg·kg<sup>-1</sup>)respectively. The results showed that, (1) The Chla, chlb, and Ch(la+b) changed little when the Cd concentration in the soil was less than 100 mg·kg<sup>-1</sup>. There was no significant difference among the treatments. When Cd concentration was more than or equal to 100 mg·kg<sup>-1</sup>, all indexes decreased significantly, Chla/b increased gradually with the increase of Cd stressful concentration; (2) Net photosynthetic rate (<em>P<sub>n</sub></em>), stomatal conductance (<em>G<sub>s</sub></em>) and transpiration rate (<em>T<sub>r</sub></em>) first increased and then decreased with the increase of Cd stressful concentration, while intercellular carbon dioxide concentration (<em>C<sub>i</sub></em>) increased significantly; (3) The photochemical quenching coefficient (qP) and electron transfer rate (ETR)&nbsp; decreased gradually with the increase of Cd stressful concentration, while the non-photochemical quenching coefficient (NPQ) increased with the increase of Cd stress concentration. It indicated that the photosynthetic mechanism of leaves was damaged; the maximum photochemical quantum yield (Fv/Fm) and potential activity (Fv/Fo) of PSII decreased gradually with the increase of Cd stress concentration. When Cd concentration was more than 50 mg·kg<sup>-1</sup>, the decrease was very obvious, showing photoinhibition. The concentration of Cd below 50 mg·kg<sup>-1</sup> had little effect on photosynthesis. These results provide a theoretical application by using these two species of <em>Rorippa</em> to ecological restore the Cd contaminated farmland and abandoned mines.</p> Yuan Yun- Ning Wang Lin- Yu Chen Hao Xu Yu- Feng ##submission.copyrightStatement## 2020-11-04 2020-11-04 32 42 10.9734/bji/2020/v24i630120 Impact of Drifts Resulting from Pesticide Application on Soil Microorganisms around Waste Receptacles in Port Harcourt City, Nigeria https://journalbji.com/index.php/BJI/article/view/30118 <p>Pesticides are toxic substance used to reduce or kill pests but the deposits on soil environment can remain there for long period of time causing adverse effects on soil microorganisms which are responsible for soil health conditions. This study was carried out to determine the impact of pesticide drifts on soil microorganisms in a waste receptacle around Port Harcourt city. Soil samples were obtained from various depths around waste receptacles with hand auger using standard analytical procedures. Microbial analysis was done according to prescribed standard methods. Characterization and identification of the isolates were based on their cultural, morphological, and cellular characteristics. Results obtained showed that the bacterial isolates were identified as <em>Staphylococus aureus</em>, <em>Bacillus subtilis</em>, <em>Bacillus megaterium</em>, <em>Pseudomonas </em>sp and <em>Micrococcus </em>sp while fungal isolates include <em>Aspergillus niger, Penicillium </em>sp, <em>Fusarium siculi, and Aspergillus fumigatus,</em> <em>Aspergillus nidulas, Microsporium canis </em>and <em>Yeast. </em>The results of the microbial counts revealed that Total Heterotrophic Bacteria (THB) had 2.08 ×10<sup>9</sup> cfu/g at a depth of 30-45 cm while Total Heterotrophic Fungi (THF) had 6.0×10<sup>6 </sup>cfu/g before application with a mean value of 1.02×10<sup>9</sup><sub>&nbsp;&nbsp; </sub>and&nbsp; <sub>&nbsp;&nbsp;</sub>2.8 × 10<sup>6 </sup>cfu/g respectively while after application THB had 4.1 × 10<sup>8</sup> and 4.6 × 10<sup>8 </sup>cfu/g for 0-15 and 30-45 cm respectively while the THF recorded 1.0 × 10<sup>6 </sup>and 0.6 × 10<sup>6 </sup>cfu/g for 0-15 cm and 30-45 cm respectively. However there was a drastic decrease in the number of microbes in the soils after pesticide application especially at the surface soil depth of 0-15 cm. This shows that the pesticides application affected microbial population by reducing their numbers in the soil and this may in turn affect soil health and physiological status of their habitat. It is therefore recommended that proper surveillance during pesticide application should be considered to avoid drift to non-target organisms and that concentrations of pesticides to be used should be taken into account to avoid reduction in the number of microorganisms in soils because of the vital roles they play in maintaining soil health.</p> D. Momoh C. L. Eze D. N. Ogbonna ##submission.copyrightStatement## 2020-09-17 2020-09-17 1 8 10.9734/bji/2020/v24i630118 Antioxidant, Hypoglycemic and Neuroprotective Activities of Extracts from Fruits Native to the Amazon Region: A Review https://journalbji.com/index.php/BJI/article/view/30119 <p>The Amazon forest has the largest biome on the planet, and it is estimated that only 16 to 20% of the identified animal, and plant biodiversity. Considering plant diversity, we will highlight the biological properties of the fruits extracts of Arecaceae, Caryocaraceae, Malvaceae, Myrtaceae, Sapindaceae, and Solanaceae’s families due to their significant biological actions. This review presents the antioxidant, glycemic control, and neuroprotective activities from ten fruit extracts distributed in six botanical families in the Amazon region. We obtained 801 publications (described from 2010 to 2020), of which 64 articles were selected by the benchmark previously chosen. The antioxidant effect was the dominant effect observed in the studies used for this review, followed by glycemic control and protective actions in neurons. This review provides a synopsis of the recent literature exploring the extracts from native fruits to the Amazon region that could efficiently prevent pathologies associated with oxidative stress, and cellular maintenance mechanisms.&nbsp;</p> Klenicy Kazumy de Lima Yamaguchi Anderson de Oliveira Souza ##submission.copyrightStatement## 2020-10-22 2020-10-22 9 31 10.9734/bji/2020/v24i630119