Biotechnology Journal International https://journalbji.com/index.php/BJI <p style="text-align: justify;"><strong>Biotechnology Journal International (ISSN:&nbsp;2456-7051)</strong> publishes original research papers, review articles and short communications on all areas of Biotechnology including cell biology, genetics, microbiology, immunology, molecular biology, biochemistry, embryology,&nbsp; immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, pharmacogenomics, gene therapy, plant, animal, microbial and environmental biotechnology.&nbsp;The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Biotechnology Journal International 2456-7051 In vitro Evaluation of the Fungicidal Potential of Aqueous and Methanolic Extracts of Thevetia peruviana on the Development of Rigidoporus lignosus, Causal Agent of White Root rot of Hevea brasiliensis https://journalbji.com/index.php/BJI/article/view/30127 <p><strong>Aims: </strong>The objective of this work is to evaluate the effect of (aqueous (EAq) and methanolic (ME)) extracts of <em>Thevetia peruviana</em> on the <em>in vitro</em> development of <em>Rigidoporus lignosus</em>.</p> <p><strong>Study Design:</strong> A synthetic fungicide (Onazol 100), two extracts at differents concentrations: C1 = 3.5 mg / ml; C2 = 7 mg / ml; C3 = 15 mg / ml; C4 = 30 mg / ml; C5 = 50 mg / ml and C6 = 100 mg / ml for EAq; C1 = 3.5 ml/ml; C2 = 7 ml/ml; C3 = 15 ml/ml; C4 = 30 ml/ml; C5 = 50 ml/ml for ME and a negative control (T = 0 mg / ml) were used.</p> <p><strong>Place and Duration of Study:</strong> The study was conducted in pathology laboratory at University of Yaounde I and IRAD of Nkolbisson in 2018.</p> <p><strong>Methodology:</strong> Daily measurements of the pathogen's development was used to evaluate the average growth (D) of the mycelium. The inhibition percentages of the different doses of EAq, ME and ONAZOL 100 were calculated after 4 to 5 days of growth of the fungus.</p> <p><strong>Results:</strong> The major result obtained compared to the rate of rot showed that the treatments EAq, ME (C6) and ONAZOL 100 were closed. The disease rates at different concentrations of C1 to C6 were 13.91 to 100% for ME, 9.34 to 100% for EAq, and 100% for ONAZOL 100.</p> <p><strong>Conclusion:</strong> The two extracts are promising, and on small and medium scale, could be an effective and cheap formulation for the control of <em>Rigidoporus lignosus</em>.</p> Serge Bertrand Mboussi Alain Heu Abdou Nourou Kone Nsangou Martial Douanla Ajebe Godswill Ntsomboh Ntsefong Jules Patrice Ngoh Dooh Zachée Ambang ##submission.copyrightStatement## 2021-01-10 2021-01-10 1 13 10.9734/bji/2021/v25i130127 Microbial Diversity of Nigerian Sludge and Its Potential for Use as Biofertilizer https://journalbji.com/index.php/BJI/article/view/30130 <p>Sludge samples were collected from a wastewater treatment plant in Nigeria for characterization and evaluation for agricultural applications. Conventional and Molecular techniques were adopted for the isolation and identification of indigenous microorganisms and resulting isolates were characterized and identified by consulting Bergey’s manual of determinative bacteriology and subjected to further screenings to assess their biofertilizer potential using standard microbiological techniques. The viable cells obtained were enumerated and were found to be in the range of 1.03 ± 0.09 x10<sup>3 </sup>cfu/g to 7.45 ± 0.78 x10<sup>3 </sup>cfu/g for heterotrophic Bacteria and 1.63 ± 0.74 x10<sup>3 </sup>cfu/g for fungal community. The Molecular analysis carried out revealed a rich assemblage of diverse species of microorganisms with Bacteria (99.40%) being the most dominant group, followed by Fungi (0.39%) and others (0.21%). Thirty (30) isolates belonging to four (4) Phyla was recovered culturally and identified with Firmicutes 9(30%) being the most dominant group, followed by Proteobacteria 8(26.7%) and Zygomycota 1(3.33%) was the least dominant. The phosphate solubilization index range from 0.86 to 6.3 for bacterial and 2.5 to 3.8 for fungal isolates respectively. The molecular analysis also revealed microbes adept at improving soil fertility to include those in the order Rhizobiales and Actinomycetales. Although pathogens are of a concern in the land application of sludge, our findings have revealed rich microbial consortia of heterotrophic microorganisms whose beneficial attributes can be harnessed to produce nutrient rich biofertilizer and soil amendment.</p> Opeyemi Fatunla Joseph Essien Utibe Ofon Solomon Shaibu Emmanuel Dan ##submission.copyrightStatement## 2021-02-26 2021-02-26 14 22 10.9734/bji/2021/v25i130130