Biotechnology Journal International <p style="text-align: justify;"><strong>Biotechnology Journal International (ISSN:&nbsp;2456-7051)</strong> publishes original research papers, review articles and short communications on all areas of Biotechnology including cell biology, genetics, microbiology, immunology, molecular biology, biochemistry, embryology,&nbsp; immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, pharmacogenomics, gene therapy, plant, animal, microbial and environmental biotechnology.&nbsp;The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> en-US (Biotechnology Journal International) (Biotechnology Journal International) Thu, 17 Sep 2020 11:11:39 +0000 OJS 60 Impact of Drifts Resulting from Pesticide Application on Soil Microorganisms around Waste Receptacles in Port Harcourt City, Nigeria <p>Pesticides are toxic substance used to reduce or kill pests but the deposits on soil environment can remain there for long period of time causing adverse effects on soil microorganisms which are responsible for soil health conditions. This study was carried out to determine the impact of pesticide drifts on soil microorganisms in a waste receptacle around Port Harcourt city. Soil samples were obtained from various depths around waste receptacles with hand auger using standard analytical procedures. Microbial analysis was done according to prescribed standard methods. Characterization and identification of the isolates were based on their cultural, morphological, and cellular characteristics. Results obtained showed that the bacterial isolates were identified as <em>Staphylococus aureus</em>, <em>Bacillus subtilis</em>, <em>Bacillus megaterium</em>, <em>Pseudomonas </em>sp and <em>Micrococcus </em>sp while fungal isolates include <em>Aspergillus niger, Penicillium </em>sp, <em>Fusarium siculi, and Aspergillus fumigatus,</em> <em>Aspergillus nidulas, Microsporium canis </em>and <em>Yeast. </em>The results of the microbial counts revealed that Total Heterotrophic Bacteria (THB) had 2.08 ×10<sup>9</sup> cfu/g at a depth of 30-45 cm while Total Heterotrophic Fungi (THF) had 6.0×10<sup>6 </sup>cfu/g before application with a mean value of 1.02×10<sup>9</sup><sub>&nbsp;&nbsp; </sub>and&nbsp; <sub>&nbsp;&nbsp;</sub>2.8 × 10<sup>6 </sup>cfu/g respectively while after application THB had 4.1 × 10<sup>8</sup> and 4.6 × 10<sup>8 </sup>cfu/g for 0-15 and 30-45 cm respectively while the THF recorded 1.0 × 10<sup>6 </sup>and 0.6 × 10<sup>6 </sup>cfu/g for 0-15 cm and 30-45 cm respectively. However there was a drastic decrease in the number of microbes in the soils after pesticide application especially at the surface soil depth of 0-15 cm. This shows that the pesticides application affected microbial population by reducing their numbers in the soil and this may in turn affect soil health and physiological status of their habitat. It is therefore recommended that proper surveillance during pesticide application should be considered to avoid drift to non-target organisms and that concentrations of pesticides to be used should be taken into account to avoid reduction in the number of microorganisms in soils because of the vital roles they play in maintaining soil health.</p> D. Momoh, C. L. Eze, D. N. Ogbonna ##submission.copyrightStatement## Thu, 17 Sep 2020 00:00:00 +0000