Optimization of Growth Parameters for Enhancing the Production of Biosurfactants from Pediococcus pentosaceus S-2

Tamanna Kaundal

Department of Biotechnology, Panjab University Research Centre, Goswami Ganesh Dutta Sanatan Dharma College (GGDSD) College, Sector-32, Chandigarh, India.

Anjali Sharma

Department of Biotechnology, DAV College, Sector- 10, Chandigarh, India.

Navneet Batra *

Department of Biotechnology, Panjab University Research Centre, Goswami Ganesh Dutta Sanatan Dharma College (GGDSD) College, Sector-32, Chandigarh, India.

*Author to whom correspondence should be addressed.


Abstract

Numerous Lactic acid bacteria (LAB) have been found to be capable of synthesizing surface-active compounds i.e biosurfactants. These are amphiphilic compounds produced by microorganisms on their cell surface or secreted extracellularly that have a tendency to reduce surface and interfacial tension. In the present study, different process parameters including nitrogen and carbon source, pH, temperature, aeration and agitation were optimized to maximize the production of biosurfactants from Pediococcus pentosaceus S-2. Xylose (1.5%) and yeast extract (1.5%) act as better carbon and nitrogen sources respectively for the production of biosurfactants. Maximum biosurfactant yield was observed at pH 6, a temperature of 35o C, an agitation rate of 200 rpm and with inoculum size of 3%. The high yield of biosurfactants produced from Pediococcus pentosaceus S-2 by utilizing media supplemented with whey under optimized conditions.

Keywords: Biosurfactants, Lactic Acid Bacteria (LAB), optimization conditions, Pediococcus pentosaceus


How to Cite

Kaundal, T., Sharma, A., & Batra, N. (2023). Optimization of Growth Parameters for Enhancing the Production of Biosurfactants from Pediococcus pentosaceus S-2. Biotechnology Journal International, 27(2), 47–55. https://doi.org/10.9734/bji/2023/v27i2678

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