Assessment of Effects of Amended Bioremediation of Toxic Metal-polluted Soil using Organic Composites and Bacillus sp on Plant Growth Parameters
Maximus Chibuoyi Korie *
Department of Science Laboratory Technology, Imo State Polytechnic, Omuma, Imo State, Nigeria.
Ferdinand Ozurumba Uzoma Osuala
Department of Biology, Federal University of Technology, P. M. B. 1528, Owerri, Nigeria.
Jude-Anthony Nnama Ogbulie
Department of Microbiology, Federal University of Technology, P. M. B. 1528, Owerri, Nigeria.
Josephat Nwabueze Okereke
Department of Biotechnology, Federal University of Technology, P.M.B. 1528, Owerri, Nigeria.
Sylvia Onyinyechi Anyadoh-Nwadike
Department of Biotechnology, Federal University of Technology, P.M.B. 1528, Owerri, Nigeria.
Colman Chikwem Ibe
Department of Science Laboratory Technology, Imo State Polytechnic, Omuma, Imo State, Nigeria.
Moses Ekene Nwachukwu
Department of Science Laboratory Technology, Imo State Polytechnic, Omuma, Imo State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
It has become pertinent to develop improved bioremediation techniques, to avert the deleterious effects of heavy metal pollution on agricultural productivity, and safety of its produce. In this study, cow dung, poultry wastes and their composites used as organic amendments were collected and processed, while Bacillus sp used as plant growth promoting rhizosphere was isolated and identified. Then 24 kg of fertile soil was polluted by introducing 20 mL of 0.1M lead acetate solution, before bagging 5 kg of polluted soil into ten perforated nylons. Soil sample was then analyzed before and after treatment. After mixing with Bacillus sp suspension, two of the pot experiments were amended with each of 0.5 kg cow dung only, 0.5 kg poultry wastes only and 1:1 ratio of their composites. Control samples were set up without amendment, and they were bioremediated for 25 days. Following germination of sown corn seedlings, heights of their shoots and number of leaves were monitored on day 4, weeks 2, 4 and 7, before harvesting for analysis together with soil samples. Results obtained revealed that seedlings grown on treated samples as well as properties of treatment of soil were generally better than those of control samples. Highest percentage increase in organic matter content of soil was recorded in treatment with mixture of cow dung only and Bacillus sp, with 9.28% and 7.63% increase at 33.75 mg/kg and 52.55 mg/kg of lead (Pb) pollution. Seedlings grown on samples treated with poultry wastes only, recorded the highest protein content of 161.25±5.5 mg/g, 104.99±4.95 mg/g and 79.75±3.2 mg/g protein contents, at 32.75 mg/kg, 42.02 mg/kg and 52.44 mg/kg levels of Pb pollution, respectively. The amendments also reduced bioaccumulation of Pb in corn seedlings, which was in the order; stem<leaf<root. Therefore, these amendments can be exploited for improved bioremediation of heavy metals.
Keywords: Pollution, bioremediation, phytotoxicity, plant growth, chlorophyll content
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