Bioethanol Production from Various Lignocellulosic Materials by Encapsulated Saccharomyces cerevisiae NCIM 3095
Biotechnology Journal International,
Page 21-29
DOI:
10.9734/bji/2022/v26i3650
Abstract
Bioethanol has a greater promise for environmental safety and energy security than fossil fuels. The alternate source required to meet the fuel's requirements can be provided by bioethanol. Untapped sugar-rich sources, like cellulose-rich household wastes, industrial wastes, and agricultural wastes, can all be used to make bioethanol at a minimal cost. The study's objective was to determine whether saccharomyces cerevisiae cells from the encapsulated NCIM 3095 strain of Saccharomyces cerevisiae could be used to make low-cost ethanol from a variety of lignocellulosic wastes, including newspaper, banana leaves, gram straw, soybean straw, and cow dung. To reduce bacterial contamination and serve as an external growth stimulator, benzathine penicillin G and ammonium sulfate were added to each sample broth containing calcium alginate-encapsulated yeast cells. The samples were fermented for ten days. The ethanol content was evaluated every three days. The largest yield of bioethanol was produced by soybean straw (10.0%), while the lowest was by cow dung (4.0%).
Keywords:
- Cellulosic wastes
- ethanol production
- biofuel
- bioethanol
How to Cite
Bhakare, S., Wagh, M. S., Kale, P., & Gadhave, A. (2022). Bioethanol Production from Various Lignocellulosic Materials by Encapsulated Saccharomyces cerevisiae NCIM 3095. Biotechnology Journal International, 26(3), 21-29. https://doi.org/10.9734/bji/2022/v26i3650
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Khan N, Roes-Hill M, Welz PJ, Grandin KA, Kudanga T, Van Dyk JS, et al. Fruit waste streams in South Africa and their potential role in developing a bio-economy, S. Afr. J. Sci. 2015;111:5-16.
Choia IS, Leea YG, Khanalb SK, Park BJ, Bae HJ. A low-energy, cost-effective approach to fruit and citrus peel waste processing for bioethanol production, Appl. Energy. 2015;140:65-74.
Wyman CE. Twenty years of trials, tribulations, and research progress in bioethanol technology, Appl. Biochem. Biotechnol. 2001;91:5-21.
Hammond JB, Egg R, Diggins D, Coble CG. Alcohol from bananas, Bioresour. Technol. 1996;56:125-130.
Optimization of Banana Peels Hydrolysis for the Production of Bioethanol: Response Surface Methodology, Mebrahtom Gebresemati1, a*, and Alula Gebregergs2, International Letters of Natural Sciences Online: 2015-11-03 ISSN: 2300-9675. 48;53-60.
DOI:10.18052/www.scipress.com/ILNS.48.53 CC BY 4.0. Published by SciPress Ltd, Switzerland, 2015.
Bioethanol Production from Banana Peels Gaddafi I. Danmaliki* 1, Auwal M. Muhammad2, Abdullahi A. Shamsuddeen3 Bashir J. Usman4.
Production of Biofuel (Bio-Ethanol) From Fruitwaste: Banana Peels Muhamad Ariff Amir Hamzah, Azil Bahari Alias, Nor Elina Ahmad, International Journal of Engineering and Advanced Technology (IJEAT) ISSN: 2249 – 8958. 2019;9(1).
Muhammad Nasidi M, Agu R, Deeni Y, Giginyu IB, Walker G. Bioconversion of degraded husked sorghum grains to ethanol, Bioeth. 2015;2:1-11.
Yamashita Y, Sasaki C, Nakamura Y. Effective enzyme saccharification and ethanol production from Japanese cedar using various pre-treatment methods, J. Biosci. Bioeng. 2010;110:79-86.
Pramanik K, Rao DE. Kinetic study on ethanol fermentation of grape waste using Saccharomyces cerevisiae yeast isolated from toddy, IE (I) Journal. 2005;85:53-58.
Sanchez OJ, Cardona CA. Review: Trends in biotechnological production of fuel ethanol from different feedstocks, Bioresour. Technol. 2008;99:5270-5295.
Bioethanol production from banana peels using different pretreatments, 2 Yasir. Yousif, Hind S Abdulhey; 2017.
Production of bioethanol from the waste newspaper, Shruti A. Byadgi a*, P. B. Kalburgiᵇ, procedia Environmental Sciences. 2016;35:555-562.
Selection of inoculum size and Saccharomyces cerevisiae strain for ethanol production in simultaneous saccharification and fermentation (SSF) of sugar cane bagasse, Maria Carolina de Albuquerque Wanderley, Mariana Lucena Soares, and Ester Ribeiro Gouveia.
Brethauer S, Wyman CE. Review: Continuous hydrolysis and fermentation for cellulosic ethanol production, Bioresour. Technol. 2010;101:4862-4874.
Campo ID, Alegria I, Zazpe M, Echeverria M, Echeverria I. Diluted acid hydrolysis pre-treatment of agri-food wastes for bio-ethanol production, Ind. Crop. Prod. 2006; 42:214-221.
Bioethanol Production Using Saccharomyces cerevisiae with Different Perspectives: Substrates, Growth Variables, Inhibitor Reduction and Immobilization, Bhadana and Chauhan, Ferment Technol. 2016;5:2.
DOI: 10.4172/2167-7972.1000131
Bioethanol Production from Waste Banana Peel Madhumala Y1, Vijayalaxmi Naganuri2, Megha Mathad3, International Research Journal of Engineering and Technology (IRJET) E-ISSN: 2395-0056. 2020;07:SPECIAL ISSUE |.
Microscopic yeast cell counting by hemocytometer, American Society of Brewing and Subcommittee on improved Microscopic Yeast Cell Counting, Journal. 1988;46:123.
Bioethanol production from starchy part of the tuberous plant(potato) using Saccharomyces cerevisiae MTCC-170, Joginder Singh Duhan a*, Ashok kumar1, Sunil Kumar Tanwar 2., African Journal of Microbiology Research.
Guneseelan NV. Biochemical methane potential of fruits and vegetables solid waste feedstocks, Biomass Bioenerg. 2004;26:389-399.
Oberoi HS, Vadlani PV, Saida L, Bansal S, Hughes JD. Ethanol production from banana peels using statistically optimized simultaneous saccharification and fermentation process, Waste Manag. 2011; 31(7):1576-84.
AOAC (Association of Official Analytical Chemists), Official methods of analysis, 16th ed. Washington; 2001.
Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem., 1956;31: 426-428.
Branyikova I, Marsalkova B, Doucha J, Branyik T, Bisova K, Zachleder V, Vitova M. Microalgae-novel highly efficient starch producers, Biotechnol. Bioeng. 2011;108: 766-776.
Takeshita T, Ota S, Yamazaki T, Hirata A, Zachleder V, Kawano S. Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions, Bioresour Technol. 2014; 158:127-34.
Gera R, Dhamija SS, Gera T, Singh D. Intergeneric ethanol producing hybrids of thermotolerant Kluyveromyces and non-thermotolerant Saccharomyces cerevisiae, Biotechnol Lett. 1997;19(2): 189-194.
Larsson S, Palmqvist E, Hahn-Hagerdal B, Tengeborg C, Stenberg K, Zacchi G, et al. The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microb. Technol. 1999; 24:151-15.
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